A host cell membrane protein, golgin-97, is essential for poxvirus morphogenesis |
| |
Authors: | Alzhanova Dina Hruby Dennis E |
| |
Affiliation: | Department of Microbiology, Oregon State University, 220 Nash Hall, Corvallis, OR 97331, USA. |
| |
Abstract: | Acquisition of the membrane and genome encapsidation is an important step in the replication of enveloped viruses. The biogenesis of the poxviral primary membrane and the core as well as the mechanisms of their maturation are poorly understood. Using RNA interference approach, we demonstrate that a cellular trans-Golgi network membrane protein, golgin-97, is essential for virus replication. Analysis of the virion morphology in the cells depleted of golgin-97 shows that the protein is required for the virus morphogenesis and, in particular, for the formation of the first infectious virus form, mature virus, but not its precursor, immature virus. This suggests that golgin-97 may be involved in the maturation of the virus core and, potentially, the virus membrane. |
| |
Keywords: | Poxvirus Vaccinia IMV MV Golgin-97 TGN RNAi |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|