人参皂甙Rh2对人脑恶性胶质瘤SHG-44细胞抗肿瘤作用的蛋白质组学分析

目的：利用蛋白质组学研究技术分离、鉴定人脑恶性胶质瘤SHG-44细胞经人参皂甙Rh2（G-Rh2）作用后差异表达蛋白，探讨G-Rh2抗胶质瘤作用机制。方法：提取32 μmol?L-1 G-Rh2处理72 h后的SHG-44细胞及空白对照组细胞总蛋白；通过双向凝胶电泳分离蛋白质
，选取2D图谱中差异表达≥1.5倍且P<0.05的蛋白质斑点，使用基质辅助激光解析离子飞行时间质谱（MALDI-TOF-MS）进行肽指纹图谱（PMF）鉴定。结果：与空白对照组比较，SHG
-44细胞经G-Rh2作用后，双向电泳发现了20个差异蛋白质点，其中16个蛋白质点表达下调，4蛋白质点上调。质谱分析了前5个差异显著的下调蛋白质点，分别为丝切蛋白1（cofilin 1）、磷酸甘油酸激酶（phosphoglycerate kinase,PGK）、过氧化物氧化还原酶1（peroxiredoxin 1,Prx 1）、热休克蛋白（heat shock proteins，HSP）和增殖细胞核抗原（proliferation cell nuclear antigen,PCNA）。结论：差异表达的蛋白质可能参与了G-Rh2对人脑恶性胶质瘤的抗肿瘤作用。

Proteomic analysis of ginsenoside-Rh2 on inhibition of human glioma cell line SHG-44

Objective To explore the mechanism of ginsenoside-Rh2(G-Rh2)on inhibition of glioma by identifying differential proteins with proteomic technique.Methods The total proteins were extracted from SHG-44 ceils treated with 32 μmol·L~(-1) G-Rh2 for 72 h and the cells in control group,then were subjected to two-dimensional gel electrophoresis.Only spots with a fold change equal or above 1.5 and P<0.05 were selected as differential proteins. Afterwards the differential proteins were analyzed by the matrix-assisted laser desorption/ionization time of-flight mass spectrometry (MALDI-TOF MS) for peptide mass fingerprint(PMF) identification.Results Compared with those of control group,20 differential protein spots were identified by the two dimensional electrophoresis in SHG-44 cells treated with G-Rh2,including 16 down-regulated ones and 4 up regulated ones.Five remarkably down-regulated proteins analyzed by mass spectrometry were cofilin 1,phosphoglycerate kinase(PGK),peroxiredoxin 1(Prx 1),heat shock proteins(HSP)and proliferation cell nuclear antigen(PCNA).Conclusion These differential proteins may be involved in the proliferation inhibition of human glioma cells by G-Rh2.