Comparison of Polymorphonuclear Cells from Healthy Donors and Differentiated HL-60 Cells as Phagocytes in an Opsonophagocytic Assay Using Antigen-Coated Fluorescent Beads |
| |
| Authors: | Bruno Guy, Catherine Testart, Sophie Gimenez, Violette Sanchez, Philippe Lheritier, Danielle Rossin, Mich le Mignon, Bernard Danve, Emanuelle Trannoy |
| |
| Affiliation: | Bruno Guy, Catherine Testart, Sophie Gimenez, Violette Sanchez, Philippe Lheritier, Danielle Rossin, Michèle Mignon, Bernard Danve, and Emanuelle Trannoy |
| |
| Abstract: | Polymorphonuclear cells (PMNs) from healthy donors and differentiated HL-60 cells were compared in an opsonophagocytic assay using fluorescent latex beads coated with Streptococcus pneumoniae polysaccharide conjugates. Serum-specific phagocytosis was efficiently mediated by both sources of cells, as measured by flow cytometry, but the mean number of beads ingested per cell was three- to fivefold higher when PMNs were used than when HL-60 cells were used. Nevertheless, differentiated HL-60 cells could be a convenient and standardized source of cells to evaluate the functionality of specific antibodies to vaccine candidates as a coating on fluorescent beads. |
| |
| Keywords: | |
|
|
