高血糖大鼠肝纤维肝脏氧化还原状态的影响

目的:观察高血糖对正常或伴随炎症、纤维化肝的氧化还原状态的影响。方法:将24只大鼠分为对照组、高血糖组、正常血糖 四氯化碳组、高血糖 四氯化碳组(STZ诱导高血糖,4周后用四氯化碳诱导肝纤维化),第9周处死。测量其肝组织GSH、SOD、MDA的水平。结果:未注射CCl4加高血糖组肝MDA较对照组有所增加,GSH、SOD则有一定程度的减少。CCl4组和CCl4加糖尿病组的MAD明显升高,GSH、SOD则降低,它们之间无明显差异。结论:8周的高血糖可能一定程度的改变肝脏的氧化还原状态,但这种改变不够明显。在并发肝脏炎症时,高血糖不能进一步加重肝脏的氧化打击。

Effect of hyperglycemia on liver redox homeostasis in rats with fibrosis induced by carbon tetrachloride

Objective:To investigate if hyperglycemia can affect the redox h omeostasis in the inflammatory and fibrotic liver of rat induced by carbon tetra chloride(CCl4).Methods:Normal male Sprague-Dawley rats were randomly divided int o four groups:control group(n=6),hyperglycemia group(n=5),normal blood glucose p lus CCl4group(n=6),hyperglycemia plus CCl4group(n=5).hyperglycemia induced with STZ(60 mg/kg);liver inflammation and fibrosis induced by CCl4(40%,2 ml/kg,biw)a fter hyperglycemia for four weeks.]All the rats were killed at week 9.GSH?SOD?MD A of the liver were measured.Results:The hyperglycemia group had higher level o f MDA and lower levels of GSH?SOD compared with control group,but there was no s tatistic significance between them.The CCl4 group and hyperglycemia plus CCl4 gr oup had an increased level of MDA and deceased level of GSH?SOD in liver compare d with control group or hyperglycemia group.Conclusion:Hyperglycemia for 8 weeks can change the redox homeostasis in liver,but can not aggravate the oxidative i njury in inflammatory or fibrotic liver.