低氧对发育期幼鼠大脑皮层GAP-43表达的进行性影响

目的：观察慢性间断性低氧（CIH）对发育期幼鼠大脑皮层生长相关蛋白（GAP-43）表达的进行性影响。方法：雄性21日龄SD幼鼠60只,体质量50±5g,随机均分为对照组和慢性间断性低氧组（CIH组）,每组30只,每组又分为2,4,6 w三个时间点,每个时间点10只幼鼠。CIH组每日间断缺氧8h,连续缺氧2,4,6 w。利用免疫组化方法检测幼鼠大脑皮层神经元GAP-43的表达。结果：与对照组比较,CIH组各时间点GAP-43的COD值均高于对照组对应时间点,差异具有显著性（P〈0.05）。结论：发育期幼鼠对CIH造成的脑损伤具有一定的代偿修复能力。

Progressive effects of hypoxia on the growth associated protein(GAP-43) of cerebral cortex in young rats at the growth and development period

Objective： To observe the progressive effects of hypoxia on the growth associated protein （GAP-43） of cerebral cortex in young rats at the growth and development period. Methods： Sixty healthy, male, 21-day-old Sprague-Dawley immature rats, weig-hing 50-4-5 g, were randomly averagely divided into two groups, control group and chronic intermittent hypoxia （CIH） group. Every group was divided into three time points. 2 week, 4 week and 6 week （n= 10）. CIH-handled immature rats were exposed to intermit-tent hypoxia in designed cabin 8 hours every day and the duration of hypoxia was 2 week, 4 week and 6 week. GAP-43 expression of cerebral cortex was detected by immunohistochemical staining. Results： Compared with the control group, GAP-43 expression of CIH group was significantly increased at the same time point （P〈0. 05）.Conclusion： Young rats at the growth and development pe-riod have certain capacity of compensatory repair to against chronic intermittent hypoxia.