Eicosapentaenoate对软脂酸诱导细胞株INS-1凋亡的保护作用研究

目的探讨eicosapentaenoate（EPA）对软脂酸诱导的大鼠胰岛B细胞瘤株INS．1凋亡的保护作用。方法根据不同干预条件分为空白对照组，EPA干预组，软脂酸干预组，EPA及软脂酸联合干预组，24h、48h后分别应用MTY检测各组细胞生长活力，48h同时应用分光光度法检测Caspase-3，Western blot检测Bax及SREBP1c的表达，ROS含量应用CM2H2DCFDA试剂盒检测。结果EPA及软脂酸联合干预组细胞生长活力较软脂酸干预组显著升高[24h：（37．33±1．15）OD vs（30．79±1．55）OD，P〈0．01；48h：（31．50±1．56）OD vs（23．94±1．10）OD，P〈0．01]，但低于空白对照组[24h：（37．33±1．15）OD vs（44．26±0．94）OD；48h：（31．50±1．56）OD vs（40．92±0．60）OD，P〈0．01]及EPA干预组[24h：（37．33±1．15）OD vs（44．37±0．79）OD；48h：（31．50±1．56）ODvs（39．45±3．42）OD，P〈0．01]，联合干预组细胞Caspase-3活力及ROS含量（3566．67±305．51）OD显著低于软脂酸干预组（4233．33±416．33）OD，高于空白对照组（2233．33±503．32）OD及EPA干预组（2566．67±321．46）OD。联合干预组较软脂酸干预组下调Bax及SREBP1c表达。结论EPA可以抑制软脂酸诱导的胰岛β细胞凋亡。

Eicosapentaenoate inhibits palmitic acid induced apoptosis in INS-1

Objective To study whether eicosapentaenoate can decrease the apoptosis effects in-duced by palmitic acid in INS-1 or not. Methods Based on different condition, there were four groups in this study, including control group , EPA group, palmitic acid group, combination of EPA and palmitic acid group. The grow curve was detected by MTT, and the expressions of bax were detected by Western blot.Cell apoptosis was detected by caspase-3. ROS was detected by CM2H2DCFDA kit after 48h. Result The grow curve of combination of EPA and palmitic acid group was higher than that in plamitic group[24 h :(37.33±1.15)OD vs (30. 79 ± 1.55 )OD, P < 0. 01 ;48 h:(31.50 ± 1.56)OD vs (23.94 ± 1.10)OD, P<0. 01] . Caspase-3 activity and ROS and the expression of bax and SREBP1c in combination of palmitic acid and T0901317 group were lower than those in palmitic acid group[(3566. 67 ± 305.51 )OD vs (4233. 33 ±416. 33)OD]. Conclusion EPA can inhibit apoptosis in INS-1 induced by palmitic acid.