枸杞多糖对小鼠T、杀伤T和NK细胞的免疫药理作用及对抗环磷酰胺的免疫抑制作用(英文)

枸杞多糖(LBP)5～10 mg/kg,ip,可以提高小鼠脾脏T淋巴细胞的增殖功能,增强CTL的杀伤功能,特异杀伤率由33％提高到67％.LBP 5mg/kg.ip,可以增强NK细胞的杀伤功能,杀伤率由12.4％提高到18％. LBP 5～10 mg/kg可以对抗环磷酰胺(Cy)对小鼠T、CTL和NK细胞的免疫抑制作用;其中T淋巴细胞的相对增殖指数(RPI)由33％提高到105％,Cy对CTL的抑制率由单用的51％降低到与LBP合用的19％和36％,NK细胞的杀伤率亦由Cy单用的9.5％提到15％和16％.以上结果说明LBP增强了正常小鼠和Cy处理鼠的T细胞介导的免疫反应与NK细胞的活性.

Effect of Lycium barbarum polysaccharides on the immune responses of T,CTL and NK cells in normal and cyclophosphamide-treated mice

LBP(Lycium barbarum polysacch-arides,extracted from the fruit of Lycium barbarum)5 or 10 mg/(kg·d),ip,could increase T lymphocyte proliferation.LBP 5 mg/(kg·d),ip,could enhance the cytotoxicity of CTL and NK cells.LBP 5 or 10 mg/(kg·d),ip,could antagonize the suppressive effect of cyclophosphamide(Cy)on T lymphocyte proliferation.Inhibitory rates of CTL cytotoxicity were decreased from 51 % in Cy control mice to 19 % and 36 % in LBP-treated Cy mice.Furthermore,administration of LBP 10 or 20 mg/(kg·d)restored the reduced NK cell cytotoxicity caused by Cy administration in mice.These results provide evidence of protective effect of LBP in augmenting T-cell-mediated immunity and NK cell activity in normal and cyclophosphamide treated mice.