泡状棘球蚴病宿主淋巴细胞的变化及意义

目的　为探讨泡状棘球蚴病宿主体内淋巴细胞在免疫调节和发病中的作用。方法　对泡球蚴感染BALB/c小鼠观察至 2 5周 ,在不同时间取脾制备细胞悬液 ,检测CD+ 4 ,CD+ 8细胞数量。对 2 5例泡球蚴病患者和 18例健康人群 ,用FCM分析了CD+ 3 ,CD+ 4 ,CD+ 8,CD+ 19,CD+ 3 8,CD+ 56和HLA -DR+ 细胞的变化。结果　泡球蚴感染BALB/c小鼠后 ,1～ 8周以CD+ 4 细胞为主 ,随后CD+ 4 细胞减少 ,CD+ 8细胞增加 ,2 0周后改变显著 (P <0 0 5 ) ,CD+ 4 /CD+ 8比值迅速倒置。泡状棘球蚴病患者CD+ 3细胞未发生改变 ,CD+ 4 细胞较正常对照组下降 (P <0 0 5 ) ,CD+ 8细胞上升 (P <0 0 5 ) ,使CD+ 4 /CD+ 8比值降低 (P <0 0 5 )。CD+ 56细胞较正常对照组显著性降低 (P <0 0 1) ,CD+ 19,CD+ 3 8和HLA -DR+ 细胞未发生改变 (P >0 0 5 )。结论　泡球蚴感染小鼠前 8周 ,以CD+ 4 细胞反应为主 ,具有保护性免疫。感染后期逐渐以CD+ 8细胞为主 ,使机体呈免疫抑制状态 ,有利于泡球蚴生存。泡状棘球蚴病患者机体呈免疫抑制状态 ,有利于泡状棘球蚴在体内的生长

Observation of lymphocyte from hosts with alveolar echinococcosis

Aim To explore the immunological regulation mechanism of host infected with alveolar echinococcus Methods The course of the infection was followed for 25 weeks,spleen cell from BALB/c mice infected with AE,CD + 4 and CD + 8 levels were determined by flow cytometry Expression of CD + 3,CD + 4,CD + 8,CD + 19 ,CD + 38 ,CD + 56 ,HLA DR + from 25 cases with AE was analyzed by flow cytometry Results 1-8 weeks of post infection,the CD + 4 cell showed a increase,and then decrease,which the CD + 8 cell gradually increased throughout the period after BALB/c mice was infected with AE There was a significant drop down that CD + 4/CD + 8 ratio since 20 weeks of post infection(P<0 05) CD + 4cells from patients with AE was significantly lower than that of control group(P<0 05),CD + 8 cells was significantly higher than that of control group(P<0 05) CD 56 +cells and CD + 4/CD + 8 ratio were remarkably decrease as compared with the controls(P<0 05),Conclusion CD 4 is mainly response in the early stage of infection,CD 4 response is replaced by CD 8 response in later period of infection AE patients exhibit a immune inhibition response,the immune inhibition response is very useful to patients with chronic alveolar echinococcus infection