血必净对脓毒症大鼠回肠黏膜形态学的影响

目的 观察不同给药途径及不同给药时间情况下,血必净注射液对脓毒症大鼠回肠黏膜形态学的影响.方法 91只健康Sprague-Dawley大鼠由中山大学实验动物中心提供,随机分为正常对照组(n=7)、脓毒症组(n=21)、灌胃给药+脓毒症组(预处理组)(n=21)、静脉给药+脓毒症组(预处理组)(n=21)和脓毒症+静脉给药组(治疗组)(n=21).除正常对照组外,其余各组按手术后3 h,6 h,12 h再分3组,每组7只.采用盲肠结扎穿孔法制作脓毒症模型.正常对照组不予任何处理,灌胃给药+脓毒症组于术前2 h灌胃给药(5 mL/kg血必净);静脉给药+脓毒症组于术前2 h尾静脉给药(5 mL/kg血必净);脓毒症+静脉给药组于术后2 h尾静脉给药(5 mL/kg血必净).正常对照组不作任何处理,直接留取组织标本;其余各组于术后3 h,6 h,12 h分别活杀大鼠留取回肠标本,用以进行形态学观察及回肠上皮损伤指数测定.用SPSS 13.0统计软件进行统计学分析;组间比较用方差分析,P<0.05为差异具有统计学意义.结果 预处理组及治疗组大鼠回肠上皮损伤程度均较脓毒症组减轻(P<0.01);预处理组中,静脉给药较灌胃给药的保护效果更好(P<0.05).结论 血必净注射液能减轻脓毒症大鼠回肠黏膜的损伤程度,预先静脉给药效果更佳.

Effects of Xuebijing injection on intestinal mucosal morphology in rats with sepsis

Objective To observe the effect of injecting Xuebijing via different routes, as either treatment or pretreatment, on changes in intestinal mucosal morphology in a rat model of sepsis. Method Ninety-one healthy Sprague-Dawley rats were randomly divided into five groups: 1) control group ( n = 7) , 2) sepsis group ( n = 21) , 3) intragastric pretreatment group ( n = 21) , 4) intravenous pretreatment group (n = 21) , and 5) intravenous treatment group (n = 21) . Except for the control group, the other groups were further divided into three sub-groups for assessment at 3, 6 and 12 h post-operation ( n = 7 per group) . Sepsis was induced by cecal ligation and puncture (CLP) . For the intragastric pretreatment group, Xuebijing injection (5 mL/kg) was administered via intragastric injection 2 hours before CLP. For the intravenous pretreatment group, Xuebijing injection (5 mL/kg)was administered via the caudal vein 2 hours before CLP. For the intravenous treatment group, Xuebijing injection (5 mL/kg) was intravenously infused 2 hours after CLP. The control group received no treatment. The ileum was removed from all rats for measurement. The rats were sacrificed at 3, 6 or 12 h after operation to obtain the ileum.Intestinal mucosal damage index and morphological changes in the intestinal mucosa were detected by light microscopy and electron microscopy. Analysis of variance was used to compare the groups. P -values < 0.05 were considered to indicate statistically significant differences. Results Intestinal mucosal damage was significantly reduced in and the three treatment groups compared with the untreated sepsis group (3h, F =53.35; 6h, F =74.93; 12 h, F - 171.27; P =0.000). Intestinal damage was significantly reduced in the intravenous pretreatment group compared with the intragastric pretreatment group (3 h, F = 53.35,P =0.036; 6 h, F = 74.93,P =0.039; 12 h, F = 171.27, P =0.042). Conclusions Irrespective of the route, the administration of Xuebijing protected against intestinal mucosal damage and intravenous pretreatment exerted the most effective protection against intestinal mucosal damage in this rat model of sepsis.