| 高表达低氧诱导因子-1对成骨细胞增殖及细胞周期的调控研究 |
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| 引用本文： | 杨林,聂鑫,姚宏,马东洋,邱宜农,田文艳.高表达低氧诱导因子-1对成骨细胞增殖及细胞周期的调控研究[J].临床军医杂志,2011,39(6). |
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| 作者姓名： | 杨林 聂鑫 姚宏 马东洋 邱宜农 田文艳 |
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| 作者单位： | 1. 解放军兰州军区兰州总医院 颌面外科,甘肃 兰州,730050
2. 解放军第三军医大学大坪医院野战外科研究所,重庆,400042 |
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| 基金项目： | 全军“十一五”医药卫生科研基金课题(06MA080) |
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| 摘 要： | 目的探讨低氧情况下高表达低氧诱导因子HIF-1对成骨细胞增殖及细胞周期的影响。方法获取大鼠颌面部成骨细胞,分别在低氧含量(2%O2)、常规氧含量(21%O2)及低氧含量(2%O2)+二甲氧乙二酰甘氨酸(DMOG)条件下进行培养,在不同培养时间分别检测成骨细胞表达的增殖能力、细胞周期变化及HIF-1蛋白的表达。结果在低氧情况下,成骨细胞增殖活性降低,细胞周期分析可见处于分裂期细胞减少,加入DMOG后细胞的HIF-1蛋白处于高表达,细胞增殖特性与常规氧含量相比无统计学差异。结论 DMOG可促进低氧状态下成骨细胞低氧诱导因子-1的高表达,有利于低氧状态下成骨细胞的增殖活性回复,可加快骨生长,为骨缺损的治疗开辟了新途径。
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| 关 键 词： | 低氧 增殖 成骨细胞 细胞周期 |
The regulation of overexpressiong of hypoxia-inducible factor-1 on the proliferation and cell cycle in osteoblastic cells |
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| Yang Lin,Nie Xin,Yao Hong,Ma Dong-yang,Qiu Yi-nong,Tian Wen-yan.The regulation of overexpressiong of hypoxia-inducible factor-1 on the proliferation and cell cycle in osteoblastic cells[J].Clinical Journal of Medical Officer,2011,39(6). |
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| Authors: | Yang Lin Nie Xin Yao Hong Ma Dong-yang Qiu Yi-nong Tian Wen-yan |
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| Institution: | Yang Lin1,Nie Xin2,Yao Hong1,Ma Dong-yang1,Qiu Yi-nong1,Tian Wen-yan1(1.Department of Maxillofacial Surgery,Lanzhou General Hospital of Lanzhou Command,PLA,Lanzhou Gansu 730050,China,2.Research Institute of Field Surgery,Daping Hospital of Third Military Medical University,Chongqing 400042,China) |
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| Abstract: | Objective To observe the regulatory effect of hypoxia-inducible factor-1(HIF-1) activation on the proliferation and cell cycle in osteoblastic cells with the environment of hypoxia. Methods Osteoblastic cells were derived from maxillofacial region of rat.The cells were cultured in three different conditions: hypoxia group(2% O2),normal group(21% O2) and hypoxia group(2% O2) + DMOG group.In the different time point after culture,the proliferation ability of osteoblastic cells was detected by MTT colorimetric... |
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| Keywords: | hypoxia proliferation osteoblastic cells cell cycle |
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