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| 大鼠乳腺肿瘤线粒体细胞色素b基因突变 | |
| 引用本文： | 王平忠,蒙世杰,姚养正,刘佩. 大鼠乳腺肿瘤线粒体细胞色素b基因突变[J]. 医学争鸣, 2000, 21(7): 865-868 |
| 作者姓名： | 王平忠 蒙世杰 姚养正 刘佩 |
| 作者单位： | 1. 第四军医大学唐都医院感染科,陕西,西安,710038 2. 西北大学生物学系,陕西,西安,710069 3. 陕西师范大学生命科学院,陕西,西安,710054 |
| 摘 要： | 目的 了解大鼠乳腺癌组织、癌旁组织和正常组织的线粒体细胞色素ｂ基因变异，６方法 提取细胞总ＤＮＡ，用ＰＣＲ法扩增细胞色素ｂ基因，产物用ＤＮＡ自动测序法进行序列分析。结果 癌组织发生了ｎｔ １４９３１Ｃ→Ｇ，ｎｔ１５００４Ｃ→Ｇ和ｎｔ １５４３５ Ｔ→Ｃ３处点突变。癌旁组织发生了ｎｔ１５４３６Ａ→Ｃ突变，正常组织没有突变。结论 大鼠乳腺癌组织和癌旁组织线粒体细胞色素ｂ基因存在突变。 |
| 关 键 词： | 乳腺肿瘤 线粒体DNA 细胞色素b基因 基因突变 |
Mutation of mitochondrial cytochrome b gene of rat breast cancer |
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| WANG Ping-Zhong,MENG Shi-Jie,YAO Yang-Zheng,LIU Pei. Mutation of mitochondrial cytochrome b gene of rat breast cancer[J]. Negative, 2000, 21(7): 865-868 | |
| Authors: | WANG Ping-Zhong MENG Shi-Jie YAO Yang-Zheng LIU Pei |
| Abstract: | AIM To know the variations of the cytochrome b genes in cancer tissue, paracarcinoma tissue and normal tissue. METHODS Cellular total DNA was extracted from three tissues. The cytochrome b genes were amplied with polymerase chain reaction (PCR). PCR products were analysed using DNA auto-sequencing method. RESULTS The cytochrome b gene of cancer tissue had the C to G mutation at nt 14931, the C to G mutation at nt 15004 and the T to C mutation at nt15435, respectively. The cytochrome b gene of paracarcinoma tissue had the A to C mutation at nt 15436. The cytochrome b gene of normal tissue had no mutation. CONCLUSION There may be mutations in cytochrome b genes from cancer tissue and paracarcinoma tissue. |
| Keywords: | rat breast neoplasm mitochondrial DNA cytochrome b gene sequence |
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