Regulation of Glycosaminoglycan Synthesis by Thyroid Hormone in Vitro |
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| Authors: | Terry J Smith Yoshiharu Murata Allen L Horwitz Louis Philipson and Samuel Refetoff |
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| Institution: | Thyroid Study Unit, Department of Medicine, University of Chicago, Illinois 60637;Thyroid Study Unit, Department of Pediatrics, University of Chicago, Illinois 60637 |
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| Abstract: | Human skin fibroblasts synthesize and accumulate glycosaminoglycans (GAG). Recently, we reported that fibroblasts incubated in thyroid hormone-deficient media accumulate more GAG than do cultures incubated in the same media enriched with 0.1 μM triiodothyronine (T3) (1981. Endocrinology. 108: 2397). The current study characterizes that enhanced accumulation. Confluent cultures were maintained in thyroid hormone-deficient media without or with added T3, labeled with 3H]acetate and analyzed for total 3H]GAG and 3H]hyaluronic acid content. |
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