16种常见致病菌16S rRNA区域进化树的建立

目的:通过建立16种常见致病菌的进化树,了解细菌之间的进化关系,为设计其诊断用芯片的特异探针提供理论基础.方法:常规方法提取细菌DNA,PCR对称扩增后进行DNA测序,利用BLAST和ClustalW程序分析测序结果并建立进化树.结果:20种细菌中,16种成功扩增出PCR产物,得到了理想的测序结果,同时建立了这16种细菌的进化树.结论:根据进化树所示,可以设计不同细菌的特异诊断用探针,利用芯片快速鉴定细菌.

Phyligenetic analysis on the 16S rRNA region of 16 common nosogenetic bacteria

Objective: To understand the phyligenetic relationship between different bacteria by establishing the phyligenetic tree of 16 common nosogenetic bacteria, and to provide theoretical basis for designing special probes in diagnostic microarray for bacteria. Methods: Routine method was used to extract the bacteria DNA. The DNA sequencing was performed after the polymerase chain reaction (PCR). By using the BLAST and ClustalW program, the sequencing results were analyzed and the phyligenetic tree was established. Results: Among the 20 kinds of bacteria, we obtained successful PCR results for 16 kinds of bacteria. The sequencing results and the phyligenetic tree were ideal. Conclusion: Based on the phyligenetic analysis, special probes for differentiating bacteria can be designed successfully. It is viable to identifying different bacteria through DNA microarray.