| 抗纤软肝颗粒对大鼠肝星状细胞增殖的影响 |
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| 引用本文： | 李志伟,程良斌. 抗纤软肝颗粒对大鼠肝星状细胞增殖的影响[J]. 中西医结合肝病杂志, 2010, 20(2): 95-97. DOI: 10.3969/j.issn.1005-0264.2010.02.011 |
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| 作者姓名： | 李志伟 程良斌 |
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| 作者单位： | 1. 湖北中医药大学2007级研究生班,湖北,武汉,430061
2. 湖北省中医院肝病科 |
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| 摘 要： | 目的:探讨抗纤软肝颗粒(KXRG)对大鼠肝星状细胞(HSC)增殖的影响。方法:传代培养的HSC-T6(肝星状细胞系)与KXRG(1.25mg/ml、2.5mg/ml、5mg/ml)及其药物血清(5%、10%、20%)共同培养72小时后,应用MTT法测定细胞增殖、流式细胞仪测定HSC细胞周期各时相的DNA含量。结果:KXRG及药物血清均能显著抑制HSC的增殖,使细胞周期阻滞于S期,且2.5mg/ml药物和10%药物血清作用最强(P0.01)。结论:KXRG通过抑制HSC的活化,阻滞其细胞周期的正常进行,进而抑制HSC增殖,从而起到抗肝纤维化的作用。
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| 关 键 词： | 肝纤维化 抗纤软肝颗粒/药效学 肝星状细胞 细胞周期 |
Effects of Kangxian Ruangan granule on rat hepatic stellate cell proliferation |
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| LI ZHI-WEI,CHENG LIANG-BIN.Postgraduate of Grade ,Hubei University of TCMChina. Effects of Kangxian Ruangan granule on rat hepatic stellate cell proliferation[J]. Chinese Journal of Integrated Traditonal and Western Medicine on Liver Diseases, 2010, 20(2): 95-97. DOI: 10.3969/j.issn.1005-0264.2010.02.011 |
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| Authors: | LI ZHI-WEI CHENG LIANG-BIN.Postgraduate of Grade Hubei University of TCMChina |
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| Affiliation: | LI ZHI-WEI,CHENG LIANG-BIN.Postgraduate of Grade 2007,Hubei University of TCM(Wuhan Hubei,430061)China |
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| Abstract: | Objective:To explore the effects of Kangxian Ruangan granule(KXRG)on rat hepatic stellate cells(HSC) proliferation.Methods:HSC-T6 with KXRG(1.25mg/ml,2.5mg/ml,5mg/ml)and its drug serum(5%,10%,20%)were cultivated 72 hours later,HSC cell proliferation was determinated by MTT,HSC cell cycle was detected by flow cytometry of DNA content in each phase.Results:KXRG group and drug serum could significantly inhibit the proliferation of HSC,the cell cycle was arrested in S phase,and 2.5mg/ml drug group and 10% drug ... |
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| Keywords: | liver fibrosis Kangxian Ruangan granule/pharmacodynamics hepatic stellate cell cell cycle |
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