改良纤维蛋白胶软骨细胞复合物修复软骨缺损的实验研究

目的：研究改良纤维蛋白胶细胞复合物和标准纤维蛋白胶细胞复合物修复关节软骨缺损的效果。方法：(1)分离3周龄幼兔关节软骨细胞并体外单层培养，将抑肽酶和氨甲环酸加入纤维蛋白胶(FG)中，构建标准FG细胞复合物和改良FG细胞复合物。(2)体外培养后植入动物模型体内，A，B组缺损内分别植入标准FG细胞复合物和改良FG细胞复合物，C组为空白对照。术后分期取材，对新生软骨进行组织学观察及氨基多糖含量测定。结果：改良FG细胞组新生软骨在组织学特性上与正常软骨相似，修复效果优于标准组。结论：在纤维蛋白胶中加入抑肽酶和氨甲环酸，可使FG降解速度与软骨细胞基质形成速度同步，提高了软骨修复质量。

The effects of stabilized fibrin-chondrocyte compounds on the healing defects of cartilage

Objective: To compare the effects of stabilized fibrin-chondrocyte compounds and standard fibrin-chondrocyte compounds on the healing defects of articular cartilage. Methods: The chondrocytes from articular cartilage of New Zealand rabbits 3 weeks old were isolated and monolayer cultured, then the cultured chondrocytes were seeded on the FG scaffold. Stabilized fibrin-chondrocyte compounds were made by adding aprotinin and tranexamic acid. Two defects of articular cartilage were made in the trochlear groove of each rabbit. The group A and B were filled with standard or stabilized fibrin-chondrocyte compounds respectively. The group C was left empty. The neocartilage was periodically examined roughly and histologically. In addition, the GAG content of the normal articular cartilage and the neocartilage 18 weeks after transplantation were detected. Results: The characteristics of neocartilage in group B were better than that of group A. The histological features of neocartilage (group B) were similar to that of the adjacent normal cartilage. Conclusions: The degradative speed of FG is slowed by aprotinin and tranexamic acid. The coincidence between FG degradative speed and extracellular matrix formation is kept up, and the quality of neocartilage adopting stabilized fibrin-chondrocyte compounds improves obviously.