| 软骨多糖对乳腺癌细胞CyclinD1及p21周期蛋白表达影响的研究 |
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| 引用本文： | 贾永利,张国蓉,刘艳玲,胡延勋,刘安军.软骨多糖对乳腺癌细胞CyclinD1及p21周期蛋白表达影响的研究[J].中国肿瘤,2007,16(6):436-438. |
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| 作者姓名： | 贾永利 张国蓉 刘艳玲 胡延勋 刘安军 |
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| 作者单位： | 天津科技大学食品工程与生物技术学院,天津,300457 |
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| 摘 要： | 目的]研究软骨多糖对乳腺癌细胞CyclinD1及p21周期蛋白表达的影响。方法]选用MCF-7人乳腺癌细胞系进行体外培养，采用MTT法检测细胞增殖作用。流式细胞仪检测细胞周期的变化，免疫荧光染色方法检测细胞周期蛋白CyclinD1及p21蛋白的表达率。结果]软骨多糖对MCF-7乳腺癌细胞体外生长有明显的抑制作用，且呈时间和剂量依赖性；软骨多糖使MCF-7乳腺癌细胞出现S期阻滞；软骨多糖促进CyclinD1蛋白失表达及p21蛋白过表达。
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| 关 键 词： | 乳腺肿瘤 细胞株 软骨多糖 |
| 文章编号： | 1004-0242（2007）06-0436-03 |
| 收稿时间： | 2006-09-21 |
| 修稿时间： | 2006-09-212006-11-08 |
Impact of Cartilage Polysaccharide on Expression of CyclinD1 and p21 in Breast Cancer Cells |
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| JIA Yong-li, ZHANG Guo-rong, LIU Yah-ling,et al..Impact of Cartilage Polysaccharide on Expression of CyclinD1 and p21 in Breast Cancer Cells[J].Bulletin of Chinese Cancer,2007,16(6):436-438. |
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| Authors: | JIA Yong-li ZHANG Guo-rong LIU Yah-ling |
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| Institution: | College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, China |
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| Abstract: | Purpose ] To study the effect of cartilage polysaccharide on expression of CyclinD1 and p21 in breast cancer cells. Methods] MCF-7 human breast cancer cells was cultured in vitro, cell proliferation effect was measured by MTT assay; cell cycle changes by flow eytometry; expression of CyclinD1 and p21 protein by immunofluorescent assay. Results] Growth of MCF- 7 cells in vitro was significantly inhibited by cartilage polysaccharide with a time dependent and dose dependent manner. Cartilage polysaecharide induced cell cycle block in S phase and promoted low expression of CyelinDl and over expression of p21. Conclusion] Cartilage polysaecharide plays anti-breast cancer effect with inducing apoptosis and changes of cell cycle in breast cancer cells. |
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| Keywords: | CyclinD1 p21 |
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