5-氨基乙酰丙酸介导的光动力学治疗人脑胶质瘤

目的:探讨光敏剂5-氨基乙酰丙酸介导光动力学疗法对体外培养的U251细胞株的疗效。方法:收集对数期生长的U251细胞，用台盼蓝染色细胞计数法确认细胞存活率在95%以上，接种于96孔板上，每孔含细胞数：5 000～10 000个, 并分别以不同累积能量（每孔累积能量分别为（15、30、45、60、75） J/cm2）、不同浓度的5-氨基乙酰丙酸（浓度分别为0 、0.5 、1.0 、1.5 、2.0 、2.5 、3.0mmol/L）介导PDT，光辐照后各孔补加50μL含10%新生牛血清的培养液,继续培养24 h。使用CCK-8法测定各孔细胞数并计算细胞存活率。结果：⑴ 5-氨基乙酰丙酸本身对U251细胞无细胞毒性作用。⑵单孔累积能量45 J/cm2、5-氨基乙酰丙酸浓度为2.5 mmol/L时，PDT效果最好，U251细胞生存率约为50.2%。结论: 5-氨基乙酰丙酸介导的PDT对U251细胞有杀伤作用。[关键词] 光动力学疗法；光敏剂；5-氨基乙酰丙酸；U251。

Photodynamic therapy mediated by 5-aminolevulinic acid for U251 human glioma

Objective To study the efficacy of photodynamic therapy(PDT) mediated by photosensitizer 5-aminolevulinic acid(5-ALA) for U251 cell line in vitro.Methods U251 cells at logarithmic growth were planted in 96-well plates,with 5000-10000 cells per well.Cell viability was confirmed by trypan blue stain with more than 95% viable cells.Groups of U251 cells were irritated with various accumulated energy(0,15,30,45,60,75 J/cm2) mediated by different concentrations of 5-aminolevulinic acid(0 mmol/L,0.5 mmol/L,1.0 mmo...