Influence of irradiation on the osteoinductive potential of demineralized bone matrix |
| |
Authors: | S Wientroub A H Reddi |
| |
Institution: | (1) Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel;(2) Bone Cell Biology Section, National Institute of Dental Research, National Institute of Health, Bethesda, Maryland, USA;(3) Department of Orthopaedic Surgery, Tel-Aviv Medical Centre, 6 Weizman Street, 64239 Tel-Aviv, Israel |
| |
Abstract: | Summary Samples of demineralized bone matrix (DBM) were exposed to graduated doses of radiation (1–15 Megarad) (Mrad) utilizing a
linear accelerator and then implanted into the thoracic region of Long-Evans rats. Subcutaneous implantation of DBM into allogenic
rats induces endochondral bone. In response to matrix implantation, a cascade of events ensues; mesenchymal cell proliferation
on day 3 postimplantation, chondrogenesis on day 7, calcification of the cartilagenous matrix and chondrolysis on day 9, and
osteogenesis on day 11 resulting in formation of an ossicle containing active hemopoietic tissue. Bone formation was assessed
by measuring alkaline phosphatase activity, the rate of mineralization was determined by measuring45Ca incorporation to bone mineral, and40Ca content measured the extent of mineralization; acid phosphatase activity was used as a parameter for bone resorption. The
dose of radiation (2.5 Mrad) currently used by bone banks for sterilization of bone tissue did not destroy the bone induction
properties of DBM. Furthermore, radiation of 3–5 Mrad even enhanced bone induction, insofar as it produced more bone at the
same interval of time than was obtained from unirradiated control samples. None of the radiation doses used in these experiments
abolished bone induction, although the response induced by matrix irradiated with doses higher than 5 Mrad was delayed. |
| |
Keywords: | Bone matrix Bone induction Radiation |
本文献已被 SpringerLink 等数据库收录! |
|