检测感染旋毛虫猪血清中循环抗原以诊断和监测本病的研究和应用

用目测快速双抗体夹心—ELISA法检测61头感染旋毛虫猪血清中CAg,40头(65.6±6.1％)呈阳性反应。100头正常猪均为阴性,30头感染囊虫和30头感染弓形体的猪,仅有1头感染弓形虫的猪出现轻度交叉反应,该猪未能追踪检查是否合并旋毛虫感染。对北京海淀区224头(1987年自外地引进),陕西商县50头和河南省新野县258头被屠杀的猪,进行流行病学调查,35头(6.6％)CAg显示阳性,其中71.4％(25/35)同时伴有抗体阳性。本法以TMBS为底物,不仅对人体无毒,而且不需新鲜配制,配好的底物溶液可以长期保存备用。本实验操作简便,完成全过程仅需20分钟,以目测观察结果,特别适于基层开展应用。实验中所需特异性抗体及酶的结合物,均系我室自制,价格低廉,相当稳定,在低温下保存2年,其效价不变,对人和动物均适用。

Diagnosing and Surveying Swine with Trichinosis by Circulating Antigen Detection

Sera of the infected pig with Trichinella spiralis (confirmed by muscle biopsy) were examined for circulating antigen of this parasite with fast double antibody sand with-EL IS A method; The positive rate was 65. 6i6. 1%. Control serum specimens obtained from 100 normal pigs all were negative. In another group of 60 swine sera with other infection ,30 from cysticercpsis cel-lulosae and 30 from, toxoplasmosis, only one due to the latter infection showed a weak cross reaction.A survey was made on slaughtered pigs naturally infected with T spiralis at western suburb (Hai Dian) of Beijing, Shang county of Shan Xi province, xin Ye of Henan province, with the fast sandwith-ELISA techinique. A total of 532 pigs were examined and the average prevalence rate by CAg was 35(6. 6%); and 71. 4(25/35)of them was also anti-trichinella antibody positive. 3,3' ,5,5'-tetramethylbenzidine sulphate (TMBS),a stable solution having kept for a fairly long time, and a new home-made nontoxic substrate of HRP were used in the present double antibody sandwith-ELISA techinique. The techinique oftfast-ELISA is simple,easy to perform, and could be done in a short time (20 minutes),besides it does not rquire sophisticated equipments. Hence it is convenient for epidemiological surveying in the field.