直接酶标法检测肝组织中丙型肝炎病毒抗原（HCAg－NS_3）的研究

以单克隆抗－ＨＣＶ－ＮＳ３直接酶标法对石蜡包埋肝组织中丙型肝炎病毒抗原（ＨＣＡｇ－ＮＳ３）进行测定，该抗体系应用基因重组表达丙肝抗原（ＨＣＶ－ＮＳ３区－Ｃ３３－Ｃ）免疫小鼠并通过细胞融合术后获得。４９例病毒性肝炎患者肝组织测定结果，抗－ＨＣＶ阳性组的ＨＣＡｓ－ＮＳ３检出率为５１．９％（１４／２７），抗－ＨＣＶ阴性组为１３．６％（３／２２），两组有显著性差异（Ｐ＜０．０１），以慢性重症肝炎和肝硬化患者检出率最高，ＨＣＡｇ－ＮＳ３阳性物在肝细胞的胞核或胞浆中均可见，呈棕黄色细小颗粒状，以核型居多。直接酶标法测定ＨＣＡｇ与原位杂交法测定ＨＣＶＲＮＡ的比较，其符合率为８１．６％（４０／４９）。本项技术具有简便、快捷、特异性、敏感性佳、图象清晰等优点，易于推广应用，为ＨＣＶ感染的临床和发病机理研究提供重要检测手段。

DETECTION OF HCV ANTIGEN-NS_3 IN LIVER TISSUE WITH MONOCLONAL ANTI-HCV BY DIRECT ENZYME IMMUNO-ASSAY

We developed a direct enzyme immuno-assay for the detection of HCV antigen-NS3 in formalin-paraffin-embedded liver tissue from 49 viral hepatitis patients. The monoclonal anti-HCV-NS3 was obtained from recombinant HCAg-NS3-C33-C. It has been proved that this is a rapid, simple,specific and sensitive method.In all of 49 cases with viral hepatitis, the detective rate of HCV-NS3 antigen was 34.7% (17/49),51.9%(14/27) in anti-HCV positve patients, and 13.6%(3/22) in anti-HCV negative patients. The positive stain was found more on the nucleus than that in cytoplasm, Hepatocytes with positive staining distributed sporadically within the lobule. Confirmation tests of this method showed that this technique was quite specific. This result was compared with in situ hybridization detection of HCV RNA and the corresponsible rate was 81.6%(P>0.05). The influence of HBV replication during HCV/HBV double infection was disscused.