PCR method to identify Salmonella enterica serovars Typhi, Paratyphi A, and Paratyphi B among Salmonella Isolates from the blood of patients with clinical enteric fever |
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| Authors: | Levy Haim Diallo Souleymane Tennant Sharon M Livio Sofie Sow Samba O Tapia Milagritos Fields Patricia I Mikoleit Matthew Tamboura Boubou Kotloff Karen L Lagos Rosanna Nataro James P Galen James E Levine Myron M |
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| Affiliation: | Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD 21201, USA. |
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| Abstract: | PCR methodology was developed to identify Salmonella enterica serovars Typhi, Paratyphi A, and Paratyphi B. One multiplex PCR identifies serogroup D, A, and B and Vi-positive strains; another confirms flagellar antigen "d," "a," or "b." Blinded testing of 664 Malian and Chilean Salmonella blood isolates demonstrated 100% sensitivity and specificity. |
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