Functional coupling between heterologously expressed dopamine D(2) receptors and KCNQ channels |
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Authors: | Ljungstrom Trine Grunnet Morten Jensen Bo Skaaning Olesen Søren-Peter |
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Institution: | (1) Division of Cellular and Molecular Physiology, Department of Medical Physiology, The Panum Institute, University of Copenhagen, Blegdamsvej 3C, Building 12.5, 2200 Copenhagen N, Denmark;(2) NeuroSearch A/S, Pederstrupvej 93, 2750 Ballerup, Denmark |
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Abstract: | Activation of KCNQ potassium channels by stimulation of co-expressed dopamine D2 receptors was studied electrophysiologically in Xenopus laevis oocytes and in mammalian cells. To address the specificity of the interaction between D2-like receptors and KCNQ channels, combinations of KCNQ1–5 channels and D2-like receptors (D2L, D3, and D4) were investigated in Xenopus oocytes. Activation of either receptor with the selective D2-like receptor agonist quinpirole (100 nM) stimulated all the KCNQ currents, independently of the subunit combination, indicating a common pathway of receptor-channel interaction. The KCNQ4 current was investigated in further detail and was increased by 19.9±1.6% (n=20) by D2L receptor stimulation. The effect could be mimicked by injection of GTP S and prevented by injection of Bordetella pertussis toxin, indicating that channel stimulation was mediated via a G protein of the G i/o subtype. Cells of the human neuroblastoma line SH-SY5Y were co-transfected transiently with KCNQ4 and D2L receptors. Stimulation of D2L receptors increased the KCNQ4 current (n=6) as determined in whole-cell patch-clamp recordings. The specificity of the dopaminergic activation of the KCNQ channels was confirmed by co-expression of other neuronal K+ channels (BK, KV1.1, and KV4.3) with the D2L receptor in Xenopus oocytes. None of these K+ channels responded to stimulation of the D2L receptor. In the mammalian brain, dopamine D2 receptors and KCNQ channels co-localise postsynaptically in several brain regions, so modulation of neuronal excitability by dopamine release could in part be mediated via an effect on KCNQ channels. |
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Keywords: | M-current KCNQ channels Dopamine D2 receptors Electrophysiology Xenopus laevis oocytes SH-SY5Y cells Quinpirole Bordetella pertussis toxin |
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