miR-32-5p靶向ZEB1、ZEB2基因调控乳腺癌增殖和侵袭能力的机制

目的 探讨ZEB1和ZEB2在乳腺癌中的作用及miR-32-5 p对乳腺癌调控的分子机制.方法 通过Western blot和qRT-PCR检测乳腺癌和癌旁组织中ZEB1、ZEB2和miR-32-5 p的表达,筛选出ZEB1和ZEB2高表达的乳腺癌细胞系,采用Western blot和qRT-PCR检测敲减效率.应用克...

MiR-32-5p affects breast cancer proliferation and invasion by interacting with ZEB1,ZEB2

Purpose To investigate the role of ZEB1 and ZEB2 in breast cancer,and the molecular mechanism of miR-32-5p on regulation of breast cancer.Methods The expressions of ZEB1,ZEB2 and miR-32-5p in breast cancer and adjacent tissues were detected by Western blot,and qRT-PCR,and breast cancer cell lines with high ZEB1 and ZEB2 expression were screened out,knock-down efficiency was detected by western blot and qRT-PCR.Clone formation,migration and invasion experiments were performed to detect the cell biological behavior after knocking down of ZEB1 or ZEB2.And Western blot was used to detect the expression of epithelial-mesenchymal transition(EMT)-related proteins(E-cadherin,vimentin).The interaction between miR-32-5p and 3′UTR ZEB1 or ZEB2 was detected by the dual luciferase reporter systems.Western blot,migration and invasion experiments were performed to detect the effects of miR-32-5p on EMT-related proteins expression,migration and invasion.Results ZEB1,ZEB2 and miR-32-5p were higher in breast cancer tissues than those in adjacent tissues.The expression of miR-32-5p was positively correlated with ZEB1 and ZEB2 in breast cancer tissues.MCF-7 cells had a higher ZEB1 and ZEB2 expression.When ZEB1 or ZEB2 was knocked down,the number of clones decreased,the migration and invasion capacitydecreased,E-cadherin protein increased,and vimentin protein decreased.3′UTR region of ZEB1 or ZEB2 exists miR-32-5p binding sites.Inhibition of miR-32-5p could increase migration and invasion capacity,increase E-cadherin protein,and decrease vimentin protein.The inhibition was declined when increasing the expression of ZEB1 or ZEB2.Conclusion miR-32-5p regulates the expression of E-cadherin and vimentin by targeting ZEB1 and ZEB2,and inhibits the proliferation,apoptosis,migration and invasion of breast cancer.