miR-196-5 p靶向HOXA5激活JAK/STAT通路促进皮肤鳞状细胞癌的增殖、侵袭及EMT

目的:探讨miR-196-5p对皮肤鳞状细胞癌(CSCC)增殖、侵袭和EMT的影响及其潜在的作用机制.方法:利用实时荧光定量PCR(RT-qPCR)检测miR-196-5 p在癌旁正常组织、CSCC组织、A431细胞和HaCaT细胞中的表达水平.过表达或下调miR-196-5 p在A431细胞中的表达后,通过CCK-8...

miR-196-5p promotes cutaneous squamous cell carcinoma proliferation,invasion, and EMT by activating the JAK/STAT pathway through targeting to HOXA5

Objective：To investigate the effects of miR-196-5p on the proliferation, invasion and EMT of skin squamous cell carcinoma and its potential mechanism. Methods: Quantitative real time PCR (RT-qPCR) was used to detect the expression of miR-196-5p in normal paracancer tissues, skin squamous cell carcinoma tissues, A431 cell and HaCaT cell. After the expression of miR-196-5p was overexpressed or down regulated, the effects of miR-196-5p on the proliferation ability of A431 cells were detected by CCK-8 experiment. The effects of miR-196-5p on the invasion ability of A431 cells were detected by transwell. Western blot was used to detect the effects of miR-196-5p on the expression of E-cadherin and N-cadherin in A431 cells. TargetScan predicted the action sites of miR-196-5p and HOXA5. The relationship between miR-196-5p and HOXA5 was analyzed by dual luciferase reporter gene assay. After up regulating HOXA5 expression in A431 cells, the effects of HOXA5 on A431 cell proliferation, invasion and EMT were detected. The effects of down regulation of HOXA5 on the JAK/STAT pathway was detected by western blot. After AG490 was applied to cells, the effects of down regulation of HOXA5on A431 cell proliferation、invasion and EMT were detected. Results:The expression level of miR-196-5p in cutaneous squamous cell carcinoma tissues was significantly higher than that in paracancer tissues（t=6.32，P=0.024）, HOXA5 expression significantly decreased（t=7.60，P=0.017）. Compared with HaCaT cells, miR-196-5p expression was significantly increased（t=18.58，P=0.003）, HOXA5 expression significantly decreased in A431 cells（t=19.72，P=0.003）. Overexpression of miR-196-5p promoted proliferation, invasion and EMT of A431 cells, while down regulation of miR-196-5p had an opposite effect. miR-196-5p targeted and negatively regulated HOXA5.Overexpression of HOXA5 inhibited the proliferation, invasion and EMT of A431 cells. Down regulation of HOXA5 activated the JAK/STAT pathway in A431 cell. AG490 downregulated the promotion of HOXA5 on A431 cell proliferation, invasion and EMT of A431 cell. Conclusion: miR-196-5p activates the JAK/STAT pathway by targeting HOXA5 to promote cutaneous squamous cell carcinoma proliferation, invasion and EMT.