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| 雷公藤内酯醇药物血清对体外培养的大鼠肾上腺皮质细胞分泌功能的影响 | |
| 引用本文： | 茆文杰,陈龙,杨春欣,姚明辉,赵子琴,沈忆文,周月琴,薛爱民,徐红梅,张明昌. 雷公藤内酯醇药物血清对体外培养的大鼠肾上腺皮质细胞分泌功能的影响[J]. 中西医结合学报, 2010, 8(6): 562-567. DOI: 10.3736/jcim20100609 |
| 作者姓名： | 茆文杰 陈龙 杨春欣 姚明辉 赵子琴 沈忆文 周月琴 薛爱民 徐红梅 张明昌 |
| 作者单位： | 1. 复旦大学上海医学院法医学系,上海,200032 2. 复旦大学中山医院药剂科,上海,200032 3. 复旦大学上海医学院药理学系,上海,200032 |
| 摘 要： | 目的：研究雷公藤内酯醇药物血清对体外培养的大鼠肾上腺皮质细胞分泌功能的影响。方法：将SD大鼠30只随机分成对照组、泼尼松组和低、中、高剂量雷公藤内酯醇组，相应药物灌胃7 d制备药物血清。分离正常大鼠肾上腺皮质细胞，分别加入药物血清常规培养48 h后，采用免疫组织化学法观察增殖细胞核抗原（proliferating cell nuclear antigen，PCNA）的表达，并计算PCNA阳性细胞数；透射电子显微镜观察肾上腺皮质细胞的超微结构；酶联免疫吸附测定法检测细胞培养上清液中皮质酮水平；实时荧光定量聚合酶链式反应法检测肾上腺皮质细胞3β-羟基类固醇脱氢酶（3β-hydroxysteroid dehydrogenase，3β-HSD） mRNA表达水平。结果：各个浓度雷公藤内酯醇药物血清组肾上腺皮质细胞培养上清液中皮质酮水平及细胞中3β-HSD mRNA表达水平较对照组明显升高；中、高剂量雷公藤内酯醇组PCNA阳性细胞比率高于对照组（P〈0.05），泼尼松组上清液中皮质酮水平、细胞中3β-HSD mRNA表达水平和PCNA阳性细胞比率均低于对照组（P〈0.05）。结论：雷公藤内酯醇对体外培养的大鼠肾上腺皮质细胞有刺激作用，可以促使肾上腺皮质细胞分泌皮质酮。 |
| 关 键 词： | 雷公藤内酯醇 中药化学成分 原代培养 肾上腺皮质细胞 增殖细胞核抗原 3-羟甾类脱氢酶类大鼠 |
Effects of triptolide-medicated serum on secretion function of adrenocortical cells isolated from rats |
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| Wen-jie MAO,Long CHEN,Chun-xin YANG,Ming-hui YAO,Zi-qin ZHAO,Yi-wen SHEN,Yue-qin ZHOU,Ai-min XUE,Hong-mei XU,Ming-chang ZHANG. Effects of triptolide-medicated serum on secretion function of adrenocortical cells isolated from rats[J]. Journal of Chinese integrative medicine, 2010, 8(6): 562-567. DOI: 10.3736/jcim20100609 | |
| Authors: | Wen-jie MAO Long CHEN Chun-xin YANG Ming-hui YAO Zi-qin ZHAO Yi-wen SHEN Yue-qin ZHOU Ai-min XUE Hong-mei XU Ming-chang ZHANG |
| Affiliation: | 1. Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032. China 2. Department of Pharmacy, Zhongshan Hospital, Fudan University, Shanghai 200032, China 3. Department of Pharmacology, Shanghai Medical College, Fudan University, Shanghai 200032, China) |
| Abstract: | Objective： To study the effects of triptolide-medicated serum on secretory function of adrenocortical cells isolated from rats. Methods： Thirty SD rats were randomly divided into control group, prednisone group, and low-, medium- and high-dose triptolide groups. Rats were administered with normal saline, prednisone and low-, medium- and high-dose triptolide respectively by gastrogavage to prepare sera containing drugs. Primary adrenocortical cells were isolated from normal male rats and cultured with sera containing drug for 48 hours. Expression of proliferating cell nuclear antigen （PCNA） was observed by immunohistochemical method and number of PCNA-positive cells was counted. Ultrastructure of adrenocortical cells was observed under a transmission electron microscope. Content of corticosterone in supernatant of adrenocortical cell culture was detected by enzyme-linked immunosorbent assay, and real-time fluorescence quantitative polymerase chain reaction （PCR） was employed to investigate the expression of 3β-hydroxysteroid dehydrogenase （3β-HSD） mRNA. Results： As compared with the control group, content of corticosterone in supernatant of adrenocortical cell culture and expression of 3β-HSD mRNA were significantly increased in the triptolide-treated groups, and the numbers of PCNA-positive cells were increased in the medium- and high-dose triptolide groups, however, they were decreased in the prednisone group. Conclusion： Triptolide-medicated serum can increase the secretion of corticosterone in rat adrenocortical cells in vitro. |
| Keywords: | triptolide chemical principles （TCD） primary culture adrenocortical cells proliferating cell nuclear antigen 3-hydroxysteroid dehydrogenases rats |
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