转基因细胞Tca8113/TNF-α的体外致突变性研究

目的　观察转基因细胞Tca81 1 3/TNF α的致突变作用 ,将转基因肿瘤细胞作为“瘤苗”的临床应用的安全性提供依据。方法　利用基因转染技术 ,用逆转录病毒LXSN·TNF α载体系统 ,将肿瘤坏死因子基因 (TNF α基因 )转导入人舌癌Tca81 1 3细胞 ,通过遗传毒理学Ames实验对转基因的Tca81 1 3/TNF α细胞的细胞DNA及其培养上清液进行体外的致突变试验研究。结果　 (1 )DNA组菌落数最大值 (x±s) :1 50 5± 1 1 2 / 1 60 2± 7 6 (TA97) ;34 0± 2 2 / 34 4± 3 2 (TA98) ;1 39 8± 4 6/ 1 30 8± 7 2 (TA1 0 0 ) ;2 64 0± 1 4 6/ 2 54 4± 8 2 (TA1 0 2 )。 (2 )培养上清液组菌落数最大值 (x±s) :1 4 0 5± 1 1 2 / 1 55 2± 7 6 (TA97) ;34 0± 1 0 / 36 4± 7 2 (TA98) ;1 4 5 6± 5 6/ 1 64 4± 6 2(TA1 0 0 ) ;2 54 0± 6 0 / 2 84 4± 8 2 (TA1 0 2 )。结论　TNF α基因转导Tca81 1 3细胞不会引起基因突变 ,转基因细胞作为“瘤苗”的应用初步评价为安全可靠的

MUTAGENESIS OF GENE-TRANSDUCED CELL TCA8113/TNF-α IN VITRO

Objective To investigate the safety of gene transduced Tca8113/TNF α as tumor vaccine. Methods TNF α gene was transduced into Tca8113. The mutagenesis of the DNA and supermatant of Tca8113/TNF α in vitro was tested by means of genetic toxicological techniques — the Ames assays. Results (1)?The hightest number (x±s) of the bacteria clone of the DNA groups were 150 5±11 2/160 2±7 6 (TA97); 34 0±2 2/34 4±3 2 (TA98); 139 8±4 6/130 8±7 2 (TA100); 264 0±14 6/254 4±8 2 (TA102)。(2)?The highest number (x±s) of the bacteria clone of the supernatant groups were 140 5±11 2/155 2±7 6 (TA97); 34 0±1 0/36 4±7 2 (TA98); 145 6±5 6/164 4±6 2 (TA100); 254 0±6 0/284 4±8 2 (TA102)。 Conclusion The mutagenesis of both the DNA and supermatant of transgenic cell Tca8113/TNF α was not observed. The initial experiment suggested that the application of gene transduced cell Tca8113/TNF α as tumor vaccine was basically safe and reliable.