| 重组人粒细胞集落刺激因子对阿糖胞苷诱导HL-60细胞凋亡的影响 |
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| 引用本文： | 王鈜,孙立荣,李丽. 重组人粒细胞集落刺激因子对阿糖胞苷诱导HL-60细胞凋亡的影响[J]. 中国实验血液学杂志, 2005, 13(6): 996-998 |
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| 作者姓名： | 王鈜 孙立荣 李丽 |
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| 作者单位： | 1. 青岛大学附属医院儿科,青岛,266003
2. 淄博科技职业学院,淄博,255000 |
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| 摘 要： | 为了研究重组人粒细胞集落刺激因子（rhG-CSF）对阿糖胞苷（Ara-C）诱导HL-60细胞凋亡的影响,体外培养HL-60细胞,加入rhG-CSF和（或）Ara-C,瑞氏染色观察细胞形态变化,吖啶橙/溴化乙锭双染色观察细胞凋亡情况,MTT实验检测细胞增殖活性.结果表明：加入rhG-CSF后Ara-C诱导的HL-60细胞的凋亡率升高（P＜0.01）,而MTT实验的OD值降低（P＜0.01）.结论：rhG-CSF有诱导HL-60细胞凋亡的作用,rhG-CSF可以促进Ara-c诱导HL-60细胞凋亡的作用.
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| 关 键 词： | 重组人粒细胞集落刺激因子 细胞凋亡 HL-60细胞系 |
| 文章编号： | 1009-2137（2005）06-0996-03 |
| 收稿时间： | 2004-12-13 |
| 修稿时间： | 2005-10-25 |
Effects of RhG-CSF on Apoptosis of HL-60 Cell Induced by Cytarabine |
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| WANG Hong,SUN Li-Rong,LI Li. Effects of RhG-CSF on Apoptosis of HL-60 Cell Induced by Cytarabine[J]. Journal of experimental hematology, 2005, 13(6): 996-998 |
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| Authors: | WANG Hong SUN Li-Rong LI Li |
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| Affiliation: | Department of Pediactrics, Affiliated Hospital of Qingdao University, Qingdao 266003 China. |
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| Abstract: | To investigate the effects of rhG-CSF on apoptosis of HL-60 cell induced by cytarabine (Ara-C), HL-60 cell were cultured with Ara-C, with or without rhG-CSF, and the changes of HL-60 cells were detected by morphology, including Wright's and acridine orange (AO) and ethidium bromide (EB) staining, and MTT assay. The results showed that comparing with Ara-C group, the apoptosis level of rhG-CSF + Ara-C group was enhanced (P < 0.01), and the OD value of MTT assay declined (P < 0.01). It is concluded that rhG-CSF can induce the apoptosis of HL-60 cells and enhance the apoptosis induced by Ara-C. |
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| Keywords: | rhG-CSF apoptosis HL-60 cell line |
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