| PCR DNA扩增、抗原、特异性抗体联合检测对结核性脑膜炎的诊断研究 |
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| 引用本文： | 王宁山,侯熙德.PCR DNA扩增、抗原、特异性抗体联合检测对结核性脑膜炎的诊断研究[J].卒中与神经疾病,1994(2). |
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| 作者姓名： | 王宁山 侯熙德 |
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| 作者单位： | 南京医科大学第一附属医院神经科 |
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| 摘 要： | 应用多聚酶链反应技术（ＰＣＲ）体外扩增脑脊液中结核分枝杆菌ＤＮＡ，酶斑免疫结合技术检测结核杆菌蛋白抗原及ＡＢＣ－ＥＬＩＳＡ法检测相应抗体联合检测诊断结核性脑膜炎，阳性率分别为：８３．７％（３６／４３），７６．７％（３３／４３），７４．４％（３２／４３）。讨论三者间发病不同期的关系，三者有阳性交叉现象。
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| 关 键 词： | 多聚酶链反应 结核性脑膜炎 脑脊液 抗原 抗体 |
A Study of Simultaneously Detecting PCR DNA Amplification,Antigen and Specific Antibody for Diagnosis of Tuberculous Meningitis |
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| Wang Ningshan,Hou Xide..A Study of Simultaneously Detecting PCR DNA Amplification,Antigen and Specific Antibody for Diagnosis of Tuberculous Meningitis[J].Stroke and Nervous Diseases,1994(2). |
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| Authors: | Wang Ningshan Hou Xide |
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| Abstract: | Using polymerase chain reaction(PCR)Dot immunobinding technique and ABCELISA methods for detecting Mycobacteral tuberculous DNA,antigen and antibody in cerebrospinal fluid of patients with tuberculous meningitis,The positive rates were 83.7%(36/43),76.7%(33/43),74.4%(32/43),respectively,There were positive crossways. |
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| Keywords: | Polymerase chain reaction Tuberculous meningitis Cerebrospinal fluid Antigen Antibody |
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