PLAC1/CP1蛋白在结直肠癌组织中的表达及其临床意义

目的 检测PLAC1/CP1(cancer-placenta 1)基因在结直肠癌肿瘤组织中的表达,探讨其与临床病理因素的关系.方法 免疫组化方法检测PLAC1/CP1基因在125例结直肠癌配对癌组织、癌旁组织芯片中的蛋白表达.统计学采用χ2检验.结果 PLAC1/CP1蛋白在125例结直肠癌组织中的表达率为57.6%(72/125).在原发腺癌中的表达率为56.7%(55/97),在低分化腺癌、中分化腺癌和高分化腺癌的表达分别为78.9%(15/19)、55.7%(34/61)、35.3%(6/17),差异有统计学意义(χ2=7.026,P=0.03);在TNMⅢ+Ⅳ期中的表达率为71.2%(37/52),明显高于Ⅰ+Ⅱ期的表达(40%,18/45)(χ2=9.537,P=0.002);在有淋巴结转移的原发灶中的表达率为69.6%(32/46)明显高于无淋巴结转移者的45.1%(23/51)(χ2=5.897,P=0.015);随淋巴结转移数目的增多,PLAC1/CP1在原发灶的表达显著增高(χ2=13.353,P=0.001).结论 PLAC1/CP1基因的蛋白表达与结直肠癌的组织学分化程度、TNM分期、淋巴结转移有关.

The protein expression of PLAC1/CP1 genes in colorectal carcinoma and its clinical significance

Objective To explore the protein expression of PLAC1/CP1 ( cancer-placenta 1 ) and its correlation with clinicopathological characteristics in patients of colorectal carcinoma. Methods The expression of PLAC1/CP1 gene was studied by using tissue chip and immunochemistry in 125 cases CRC tissue specimens. Data were analyzed with the x2-test or Fisher's x2 test statistic by SPSS 16. 0 software.Results The protein expression of PLAC1/CP1 gene was 57.6% (72/125) in 125 cases of CRC and 56. 7% (55/97)in 97 primary adenocarcinoma cases. That was 78. 9% (15/19)in poor differentiated colorectal primary adenocarcinoma, those were significantly higher than that of 35.3% (6/17) in well and 55.7% (34/61) in moderately differentiated adenocarcinoma ( P ＜ 0.05 ); PLAC1/CP1 protein expression was significantly higher in stage TNM Ⅲ + Ⅳ 71.2% (37/52) than in stage TNM Ⅰ + Ⅱ 40% (18/45)(P ＜ 0.05 ); PLAC1/CP1 genes expression rate was 69.6% (32/46) in these with lymphoid node metastasis and 45.1% (23/51)in patients without lymphoid node metastasis (P ＜ 0.05 ). The positive expression rate of PLAC1/CP1 increased in colorectal carcinoma with the increasing of the numbers of lymphoid node involved with metastasis( x2 = 13. 353, P = 0.001 ). Conclusions The protein expression of PLAC1/CP1 is associated with tumor differentiation, TNM stage and lymphoid node metastasis.