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| 软骨细胞培养上清液抑制血管生成的研究 | |
| 引用本文： | 和新盈,张润岐,王书文,吴庚利,曹峻岭.软骨细胞培养上清液抑制血管生成的研究[J].肿瘤研究与临床,2002,14(1):9-11. |
| 作者姓名： | 和新盈 张润岐 王书文 吴庚利 曹峻岭 |
| 作者单位： | 1. 陕西医学高等专科学校，陕西 西安 710068 2. 西安交通大学第一医院，陕西 西安 710061 3. 西安交通大学医学院地方病研究所，陕西 西安 710061 |
| 基金项目： | 陕西省卫生厅青年科研基金资助项目 ( 99-35 3) |
| 摘 要： | 目的：检测软骨细胞培养上清液中是否存在抑制血管生成的因子。方法：用酶消化、分离鸡胚关节软骨细胞，体外单层培养，收集细胞培养上清液。并将该上清液作用于小鼠种植性实体瘤S180及培养人脐静脉内皮细胞（HUVEC）上，探讨其抗血管形成作用的机制。结果：软骨细胞培养上清液可抑制内皮细胞由G1期向S期转换，使细胞停留于G0／G1期。与对照组相比，增殖指数（PI）明显下降，瘤重、肿瘤微血管密度（intratumoral microvessel density,iMVD)也显著降低。结论：软骨细胞培养上清液中具有抑制血管生成的活性因子。 |
| 关 键 词： | 软骨细胞培养 血管生成抑制因子 血管内皮细胞 肿瘤 动物实验 治疗实验 |
| 文章编号： | 1006-9801(2002)01-0009-03 |
The Investigation of Anti-angiogenesis by Chondrocyte Culture Stromal Supernatant |
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| HE Xin ying,ZHANG Run qi,WANG Shu wen,et al..The Investigation of Anti-angiogenesis by Chondrocyte Culture Stromal Supernatant[J].Cancer Research and Clinic,2002,14(1):9-11. | |
| Authors: | HE Xin ying ZHANG Run qi WANG Shu wen |
| Institution: | HE Xin ying,ZHANG Run qi,WANG Shu wen,et al.Shannxi Medical Collage |
| Abstract: | Objective:To detemine whether inhibitor of angiogenesis present or not in chondrocytes culture stromal supernatant.Methods:Chick embryo joint chondrocytes digested and isolated by enzyme were grown monolayer culture in vitro.Chondrocyte culture stromal supernatant was collected.The anti angiogenesis mechanisms of the cullture stromal supernatant was studied by the implanted sarcoma S 180 experiment and culture endothelial cell.Results:HUVEC transform G 1 phase to S phase can be decreased by Chondrocyte culture stromal supernatant.Contrast with the control groups,the proliferation indices (PI) decreased evidently,the weight of tumor and the intratumoral microvessel density (iMVD) decrase either.Conclusion:Chondrocytes culture stromal supernatant are the angiogenesis inhibitors. |
| Keywords: | Chondrocytes culture Angiogenesis inhibitors Vascular endothelial cell |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |