后海穴区CB—HRP轴浆运输追踪的神经元节段性分布及相应…

用ＣＢ－ＨＲＰ对大鼠后海穴区进行神经节段性分布的研究，在Ｌ６～Ｓ１水平标记出核穴相关运动神经元（Ｍｎ）的树突形成软膜下树突丛和室管膜下树突丛，两者均为嫌高尔基树突，有些远端树突穿越到是灰质，在背角和背连合核（ＤＣＮ，Ｓ１～２）区域内ＣＢ－ＨＲＰ标记的初级跨节感感觉纤维和标记的前终末纤维可与逆行标记的Ｍｎ树突汇聚和重叠，电针或免疫注射动物的Ｍｎ，其树突软膜下丛和室管膜下丛减少，结果提示后海穴区的神经

Spinal segment distribution of neural innervation related houhai acupoint--studied by CB-HRP tracing method focused on observation of the dendrites of spinal motor neurons]

The spinal segment distribution of neural innervation related Houhai acupoint in rats has been investigted by using horseradish peroxidase conjugated cholera toxin B subunit (CB-HRP) tracing technique. Spinal motoneurons (Mn) in L6-S1 were labeled retrogradely by CB-HRP, and their features have been characterized: 1) their marginal plexus of the dendrites radially projected to the lateral and/or anterior funiculus and their distal portions forming subpial dendritic plexus; 2) the medial dendrites ran along the ventral margin of the gray commissure and their distal portions surrounded the central canal' forming a subependymal dendritic plexus. Both of them are Golgi-phobic. Some distal branches of dendrites penetrated to the contralateral gray matter. The dendritic arbors of the labeled motoneurons in the group of electro-acupuncture or group of acupoint-inoculation with sheep red blood cells contracted in comparison with that in the normal control group. The primary sensory transganglionic labeled fibers and preterminal fibers revealed by CB-HRP in the dorsal horn and dorsal commissural nucleus of the spinal cord (L5-S2) converged and overlaped with the dendrites of retrogradely labeled motoneurons. The results indicated there is a reflect circuit of afferent-integration-efferent neuro-network in the spinal cord related Houhai acupoint. The Mns retrogradely labeled by CB-HRP in the L6-S1 segments might be involved in Houhai acupuncture.