甘糖酯对血小板与内皮细胞功能的影响

研究了类肝素物质甘糖酯对血小板膜表面GMP－140分子表达的影响以及对人脐静脉内皮细胞和平滑肌细胞生长的作用。结果显示：凝血酶可刺激血小板膜表面GMP-140的表达，甘糖酯明显抑制凝血酶的这种刺激作用，其抑制作用强于消炎痛。甘糖酯不影响平滑肌细胞的生长，但在很小剂量下即明显促进内皮细胞生长。提示甘糖酯可能成为一种很有前途的抗凝抗栓药物。

Effects of PGMS on the Functions of Human Platelets and Endothelial Cells

OBJECTIVE: To study the effects of Propylene glycol mannte sulfate (PGMS), a heparin-like substance on the functions of platelets and endothelial cells. METHODS: Using 125I labeled monoclonal antibody to measure the expression of GMP-140 on the surface of human platelets; and using naphthol blue black stain method to study the growth of human umbilical vein endothelial cells (HUVEC) and human umbilical vein smooth muscle cells (HUVSMG). RESULTS: PGMS significantly inhibited thrombin stimulated expression of GMP-140 on the surface of human platelets. IC50 was 127 micrograms/ml. Indomethacin also inhibited GMP-140 expression, but its effect was weaker than that of PGMS with the IC50 of 502 micrograms/ml. PGMS did not affect the growth of HUVSMC. On the contrary, it significantly promoted the growth of HUVEC at very low concentration (1.6 micrograms/ml). CONCLUSIONS: PGMS showes anticoagulative effects on both platelets and endothelial cells and it might well be developed as a new antithrombotic and anticoagulateve agent.