| X染色体长臂末端28个新序列标签位点的分离 |
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| 引用本文： | 秦学斌 孔建. X染色体长臂末端28个新序列标签位点的分离[J]. 中国医学科学院学报, 1997, 19(1): 1-5 |
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| 作者姓名： | 秦学斌 孔建 |
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| 基金项目： | 国家自然科学基金!39392900 |
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| 摘 要： | 用脉冲场凝胶电泳分离回收覆盖脆性X位点的酵母人工染色体YAC209G4中475kb片段，经MboⅠ完全酶解为平均长度400bp的片段，与pBSⅡKS质粒载体重组，共得到3500个重组作，筛选出60个含单拷贝序列插入片段的克隆。经序列测定，并与基因数据库分析比较，其中28个序列被基因数据库接受为新的序列标签位点，GenBank收录号为U26560-U26587。根据其中3个新序列标签的核普酸顺序设计PCR引物，在人基因组DNA中扩增出相应的特异片段。
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| 关 键 词： | 序列标签位点 脆性X综合征 |
Isolation of 28 New STSs at Xq27. 3 |
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| Qin Xuebin, Kong Jian, Huang Tao ,Zhang Jun, Shen Yan, et al. Isolation of 28 New STSs at Xq27. 3[J]. Acta Academiae Medicinae Sinicae, 1997, 19(1): 1-5 |
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| Authors: | Qin Xuebin Kong Jian Huang Tao Zhang Jun Shen Yan et al |
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| Affiliation: | National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS, Beijing. |
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| Abstract: | A plasmid sublibrary of the 475 kb insert of YAC209G4 was constructed by using pBS II KS vector. The library of 3,500 clones having 100-600 bp inserts was screened with the probe of the 475 kb insert blocked with competitor DNA. Sixty unique single copy clones were found and sequenced. Checking with GenBank, 28 new STSs were obtained. Genbank accession numbers are U26560-26587. Three STSs were tested by PCR with appropriate primers using human genomic DNA as template and showed specific amplification bands as expected. |
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| Keywords: | sequence-tagged site fragile X syndrome |
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