羊水细胞培养及染色体制备方法的探讨

目的:探讨羊水细胞培养及染色体制备方法。方法:孕15～29周具有产前指征的50例孕妇,抽取羊水细胞培养6～7天,换液第二天取出观察,见有许多大而亮圆的分裂期细胞时,加入秋水仙素继续培养1小时后收获、低渗、固定、制片、消化、染色。结果:50例羊水细胞培养全部成功,成功率100%。有49例获得满意的染色体分裂相。结论:该方法细胞培养成功率高、有效分裂相多、实验稳定、易于操作、成功率高。

Investigation of the method about amniotic fluid cell culture and chromosome preparation

Objective:To investigate a convenient,stable,reliable and effective method about amniotic fluid cell culture and chromosome preparation.Methods:50 prenatal women with 15~29 gestational weeks were selected from the out-patient department of our hospital,whose amniotic fluid cells were cultured for 6~7 days.Then we observed after the second day of changing the liquid,and saw many big,bright and round dividing phase cells.Harvesting,lowwing permeability,fixing,moviemaking,digesting and staining were performed respectively after joining colchicine to continue to culture one hour.Results:50 cases of amniotic fluid cell culture were all successful,the achievement rate was 100 per cent.49 cases were satisfied with the splitting phase of the chromosome.Conclusion:We can get a high achievement rate,effective splitting phase,experimental stability,ease of operation with this cell culture method.