血清IL-17在结直肠癌中的表达及临床意义

目的研究血清IL-17在结直肠癌患者术前、术后1d及术后7d不同时段中的表达水平及其临床意义。方法应用双抗体夹心ABC-ELISA法检测20例健康志愿者（对照组）和55例结直肠癌患者术前（A组）、术后1d（B组）、术后7d（C组）血清中的IL-17浓度,分析其与结直肠癌临床病理特征的关系和术前、术后不同时段的变化。结果 A组、B组血清IL-17表达水平明显高于对照组（P〈0.05）,C组血清IL-17浓度高于对照组,但差异无统计学意义（P〉0.05）。C组血清IL-17表达水平明显低于A组与B组（P〈0.05）,B组血清IL-17浓度高于A组,但差异无统计学意义（P〉0.05）。IL-17的表达水平与肿瘤浸润深度、肿瘤分化程度、TNM分期、有无肿瘤神经束侵犯有关（P〈0.05）,T4b患者IL-17浓度明显高于T1～3、T4a患者（P〈0.05）,低分化患者IL-17浓度明显高于中、高分化患者（P〈0.05）,Ⅲ期患者血清IL-17浓度明显高于Ⅰ～Ⅱ期患者（P〈0.05）,有肿瘤神经束侵犯患者IL-17浓度明显高于无侵犯患者（P〈0.05）。IL-17表达水平与患者性别、年龄、肿瘤大小、肿瘤部位及脉管有无侵犯等因素无关（P〉0.05）。结论 IL-17在结直肠癌患者血清中高表达,与肿瘤浸润深度、肿瘤分化程度、TNM分期有关,其可能在结直肠癌的发生、发展中起重要的作用。

The expression and clinical significance of serum IL-17 in colorectal cancer

Objective To study the expression level and clinical significance of serum IL-17 in colorectal cancer patients at different time：before surgery, 1 day and 7 days after operation. Methods Detected the concentration of serum IL-17 of 20 healthy volunteers（the control group） and 55 cases of colorectal cancer patients at pre-operative（group A）, 1 day after operation（group B）, 7 days after operation（group C） by double antibody sandwich ABC-ELISA, and analyzed the relationship between IL-17 and clinical pathological characteristics of colorectal cancer, the preoperative and postoperative changes at different time. Results The expression level of serum IL-17 of patients in group A and group B was significantly higher than the control group（P〈0.05）, and the concentration of serum IL-17 of group C was also higher than that of the control group, but there was no statistically significant difference（P〈0.05）. The expression level of serum IL-17 of group C was obviously lower than that of group A and group B（P〈0.05）,while the concentration of serum IL-17 of group B was higher than that of group A, which had no statistically significant difference（P〈0.05）. The expression level of IL-17 was associated with infiltration depth of tumor, differentiation degree of tumor, TNM stages and whether it related to nerve tract invasion or not（P〈0.05）, and the concentration of serum IL-17 in T4b patients was significantly higher than T1～3,T4a patients（P〈0.05）,and it was also significantly higher in the patients with low differentiation than the patients with moderate differentiation and high differentiation（P〈0.05）, the concentration of serum IL-17 in the patients with stage Ⅲ was higher than that of patients stage Ⅰ～Ⅱ, and the concentration of patients with tumor nerve bundle invasion was significantly higher than that of patients with no invasion（P〈0.05）. The expression level of IL-17 had nothing to do with such factors as patient gender, age, tumor size, tumor lo