L-选择素、异黏蛋白mRNA在胃癌中的表达及意义

目的：在基因水平检测胃癌组织L-选择素和异黏蛋白（metadherin,MTDH）的表达情况,并分析二者与胃癌临床病理特征的关系。方法：应用RT-PCR法检测90例胃癌组织和55例癌旁正常组织L-选择素和MTDH mRNA的表达。结果：胃癌组织L-选择素和MTDH mRNA的相对表达量明显高于癌旁正常组织（P〈0.05）。L-选择素、MTDH mRNA的表达与胃癌的分化程度、浸润深度、淋巴转移、TNM分期有关（P〈0.05）,与性别、年龄无关（P〉0.05）。胃癌组织L-选择素、MTDH mRNA的表达呈正相关（r=0.369,P〈0.05）。结论：L-选择素和MTDH与胃癌的发生、发展密切相关,可作为评估恶性肿瘤转移潜能和预后的指标。

EXPRESSION AND SIGNIFICANCE OF L-SELECTIN AND MTDH IN GASTRIC CARCINOMA

Objective：To detect the expression of L-selectin and metadherin（MTDH） at genetic level in gastric carcinoma and analyze the relationship between their expression and clinical pathological characteristics of gastric carcinoma.Methods：The expression of L-selectin and MTDH mRNA in 90 cases gastric carcinoma and 55 cases corresponding nontumor normal tissues were detected by RT-PCR.Results：The relative expression of L-selectin and MTDH mRNA in gastric carcinoma were all obviously higher than that in corresponding non-tumor normal tissues（P 0.05）.The expression of L-selectin and MTDH mRNA in gastric carcinoma were related to differentiation degree,infi ltration depth,lymph node metastasis and TNM stage（P 0.05）;but no related to age and sex（P 0.05）.In gastric carcinoma,the MTDH mRNA expression was positively related to L-selectin（P 0.05）.Conclusions：L-selectin and MTDH is closely related to development of gastric carcinoma,and can be acted as index for evaluating the metastatic potential of malignant tumor and prognosis.