AFP、GGT、GPC3联合检测在原发性肝癌诊断中的应用

目的:探讨血清肿瘤标志物甲胎蛋白(AFP)、谷氨酰转肽酶(GGT)、磷脂酰肌醇蛋白聚糖3(GPC3)单独及联合检测对原发性肝癌(PHC)的诊断价值。方法:承德市中心医院2009年8月-2011年7月收治的55例PHC患者,采用电化学发光法检测AFP水平、速率法检测GGT水平、ELISA法检测GPC3水平。结果:PHC患者血清GPC3、GGT和AFP阳性率分别为81.8%、90.9%、74.5%,明显高于健康人群,差异有统计学意义(P0.01)。单独检测,GPC3检出率最高;两个指标联合检测,AFP+GPC3最优;三项指标联合检测敏感性及准确度虽有所升高,但特异度明显较低。结论:血清肿瘤标志物AFP、GGT、GPC3对PHC有一定的诊断价值;GPC3和AFP联合检测可明显提高PHC的诊断率,特别是AFP阴性或AFP低浓度的PHC患者。

APPLICATION OF COMBINING DETECTION OF AFP,GGT AND GPC3 IN DIAGNOSIS OF PRIMARY HEPATIC CANCER

Objective:To investigate the diagnostic value of separating and combining detection of serum tumor marker alpha-fetoprotein(AFP),glutamyltranspeptidase(GGT) and glypican 3(GPC3) in diagnosis of primary hepatic cancer(PHC).Methods:Electrochemiluminescence was used to detect AFP,velocity method to detect GGT,ELISA to detect GPC3 in serum of 55 PHC patients from 2009.8 to 2011.7.Results:The level of GPC3,GGT and AFP in serum of PHC patients were respectively 81.8%,90.9% and 74.5%,which were obviously higher than healthy persons(P<0.01).Separating detection,the detection rate of GPC3 was the highest;2 indexes combining detection,AFP+GPC3 was the best;the sensibility and accuracy of 3 indexes combining detection elevated,but specificity decreased.Conclusions:GPC3,GGT and AFP have fairly diagnostic value for PHC.Combining detection of GPC3+AFP can obviously increase the diagnostic rate of PHC,especially for patients that AFP is negative or low concentration.