益智仁挥发油薄层指纹图谱研究 |
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引用本文: | 龙兰艳,胡昌江,李文兵,李倩,吴文辉.益智仁挥发油薄层指纹图谱研究[J].成都中医药大学学报,2012,35(1):58-59,73. |
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作者姓名: | 龙兰艳 胡昌江 李文兵 李倩 吴文辉 |
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作者单位: | 龙兰艳 (成都中医药大学药学院,四川成都,611137) ; 胡昌江 (成都中医药大学药学院,四川成都,611137) ; 李文兵 (成都中医药大学药学院,四川成都,611137) ; 李倩 (成都中医药大学药学院,四川成都,611137) ; 吴文辉 (成都中医药大学药学院,四川成都,611137) ; |
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基金项目: | 国家自然科基面上项目(批准号:30973941) |
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摘 要: | 目的:建立益智仁挥发油薄层指纹图谱,为其鉴别和质量评价提供依据。方法:采用薄层色谱(TLC)方法,建立了益智仁挥发油的薄层指纹图谱。结果:确定益智仁挥发油薄层条件为:预制硅胶G高效薄层板,以石油醚(60℃~90℃)-环己烷-乙酸乙酯(8∶1∶1)为展开剂,点样量为2μL。显色条件为:喷10%硫酸乙醇溶液,于105℃烘5 min,置紫外光灯下(365 nm)观察,得到益智仁挥发油的薄层色谱指纹图谱。结论:所建立的方法具有简便,专属,稳定,直观,可重复的特点,可用于益智仁的鉴别和质量的评价。
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关 键 词: | 益智仁 挥发油 薄层指纹图谱 |
Research on Thin-Layer Chromatography Fingerprint of Volatile Oil from Alpinia Oxyphylla Miquel |
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Institution: | LONG Lan-yan,HU Chang-jiang,LI Wen-bing,et al(Chengdu University of T.C.M,Chengdu,611137,China) |
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Abstract: | Objective:To establish thin-layer chromatography(TLC) fingerprint of Volatile Oil from Alpinia oxyphylla Miquel for providing the reference for its identification and quality evaluation.Methods:TLC fingerprint chromatograms were established by TLC fingerprint of Volatile Oil from Alpinia oxyphylla Miquel.Results:Chromatographic conditions were as follows: stationary phanol-precoated HPTLC silica gel 60 plate;developing solvent:petroleum ether(60 ℃-90 ℃)-cyclohexane-ethylacetate(8∶ 1∶ 1);The optimum dropping amounts was 2 microlitres;derivative reagent:10%H2SO4 ethanolic solution,heating at 105℃ for 5 min and observing the fluorescent chromatogram in a UV cabinet at 365 nm,then establishing TLC fingerprint of Volatile Oil from Alpinia oxyphylla Miquel.Conclusion:Themethod has been proved to be feasible for the identification of this crude drug. |
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Keywords: | Alpiniao oxyphylla Miq volatile oil TLC fingerprint spectrum |
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