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Effects of Lymphocyte Isolation and Timing of Processing on Detection of CD127 Expression on T Cells in Human Immunodeficiency Virus-Infected Patients |
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| Authors: | Jeanette Higgins Julia A. Metcalf Randy A. Stevens Michael Baseler Martha C. Nason H. Clifford Lane Irini Sereti |
| Affiliation: | AIDS Monitoring Laboratory, Clinical Services Program, Science Applications International Corp., Frederick,1. National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland2. |
| Abstract: | Decreases in the detection of CD127 expression on T cells of human immunodeficiency virus-infected patients by flow cytometry can occur by delayed processing or by peripheral blood mononuclear cell isolation and cryopreservation. These observations should be considered in the interpretation of functional studies and the planning of multicenter clinical trials. |
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