| INVASION OF SPHEROID OF MURINE LUNG ADENOCARCINOMA (LA_(795)) CELLS INTO EMBRYONIC CHICK HEART FRAGMENTS |
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| 引用本文： | 许三多,高进. INVASION OF SPHEROID OF MURINE LUNG ADENOCARCINOMA (LA_(795)) CELLS INTO EMBRYONIC CHICK HEART FRAGMENTS[J]. 中国癌症研究, 1992, 4(4): 17-22. DOI: 10.1007/BF02996397 |
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| 作者姓名： | 许三多 高进 |
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| 作者单位： | Department of Cell Biology，Beijing Medical University，Beijing，Department of Pathology，Institute of Basic Medical Sciences，Chinese Academy |
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| 摘 要： | An in vitro spheriod invasion model of tumor cell was established by using murine lung adenocarcinoma cell line (LA795) and precultured embryonic chick heart fragment (PHF). The spheroid of LA795 cells were prepared by incubating a suspension of trypsinized LA795 cells on a gyratory shaker. Spheroid aggregates of LA795 cells in diameter of 0. 2 mm were selected and confronted with PHF (diameter of 0. 4 mm) on semi- solid medium for 3 - 4 hours, then, individual confronting pain were transferred into fluid medium for further co-culture on gyratory shaker. After 1, 3, 5 and 7 days, multiple confronting pairs were processed for histological and ultrastructural study. The Invasive capacity and the invasion process of LA795 cells were examined and observed. The results demonstrated that LA795 cell line has a high capacity of invasion and high malignancy in vitro. This spheroid Invasion model is very useful for studying mechanism of Invasiveness of tumor cells in vitro.
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Invasion of spheroid of murine lung adenocarcinoma (LA795) cells into embryonic chick heart fragments |
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| Sanduo Xu,Jin Gao. Invasion of spheroid of murine lung adenocarcinoma (LA795) cells into embryonic chick heart fragments[J]. Chinese Journal of Cancer Research, 1992, 4(4): 17-22. DOI: 10.1007/BF02996397 |
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| Authors: | Sanduo Xu Jin Gao |
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| Affiliation: | Xu Sanduo Gao JinDepartment of Pathology,Institute of Basic Medical Sciences,Chinese Academy of Medical SciencesDepartment of Cell Biology,Beijing Medical University,Beijing |
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| Abstract: | An in vitro spheriod invasion model of tumor cell was established by using murine lung adenocarcinoma cell line (LA795) and precultured embryonic chick heart fragment (PHF). The spheroid of LA795 cells were prepared by incubating a suspension of trypsinized LA795 cells on a gyratory shaker. Spheroid aggregates of LA795 cells in diameter of 0. 2 mm were selected and confronted with PHF (diameter of 0. 4 mm) on semi- solid medium for 3 - 4 hours, then, individual confronting pain were transferred into fluid medium for further co-culture on gyratory shaker. After 1, 3, 5 and 7 days, multiple confronting pairs were processed for histological and ultrastructural study. The Invasive capacity and the invasion process of LA795 cells were examined and observed. The results demonstrated that LA795 cell line has a high capacity of invasion and high malignancy in vitro. This spheroid Invasion model is very useful for studying mechanism of Invasiveness of tumor cells in vitro. |
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| Keywords: | Invasion spheroid lung adenocarcinoma precultured chick heart fragment (PHF) organ culture. |
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