蝇蛆壳提取壳聚糖的综合评价

目的 壳聚糖具有良好的水溶性、生物相容性、生物降解性、成膜性及止血、愈创、消炎等作用,在医用生物材料应用方面具有广阔前景.蝇蛆的皮中含有大量壳聚糖成分,是获得较纯壳聚糖的方式之一.对蝇蛆提取的壳聚糖创伤敷料进行综合评价.方法 按照国家统一标准对蝇蛆壳聚糖进行理化分析(相对分子质量、脱乙酰度和重金属含量)、抑菌、皮肤致敏、细胞毒性和溶血实验.结果 蝇蛆壳聚糖的相对分子质量为266 ku,脱乙酰度为75％,重金属含量小于10-5,细胞毒性总评级为1级,具有较轻细胞毒性.致敏和溶血实验结果表明,蝇蛆壳聚糖材料不具有致敏反应,材料的溶血率为0.769％,小于5％,即没有溶血现象.此外,蝇蛆壳聚糖还具有抑菌作用.结论 蝇蛆壳聚糖具有较好的综合特性,可以满足生物医药方面的要求.

Evaluation of the chitosan from flies maggots shell

Objective Chitosan has been widely studied in the fields of biomedical materials and tissue engineering,due to its good water solubility,biocompatibility,biodegradability,film-forming property,hemostasis,wound healing and anti-inflammatory effects.Flies maggot shell contains much chitosan,which is one of the excellent raw materials.The aim of this study is to provide integral biological assessment on chitosan trauma dressing.Methods Chitosan samples were subjected to physicochemical analysis (relative molecular mass,degree of deacetylation,and content of heavy metal),bacteriostatic test,skin sensitization,cytotoxicity tests as well as hemolysis test according to national standard of China.Results The relative molecular mass of chitosan was 266 ku,the degree of deacetylation of chitosan was 75％,and the content of heavy metal was less than 10-5.The cytotoxicity level was evalnated at the first level which indicating the low cell toxicity.Skin sensitization test showed that no sensitization reaction was practically detected.The hemolysis rate was 0.769％,which was less than 5％,indicating zero hemolysis.Conclusions Chitosan has good character,which may meet the requirements of biological and medical application.