苯妥英钠致大鼠骨质疏松模型的建立及维生素D预防作用的观察 |
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引用本文: | 姚文思,刘香君,丁春慧,孙梦雪,秦园园,顾兵,燕宪亮. 苯妥英钠致大鼠骨质疏松模型的建立及维生素D预防作用的观察[J]. 国际病理科学与临床杂志, 2016, 0(4): 433-438. DOI: 10.3978/j.issn.2095-6959.2016.04.017 |
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作者姓名: | 姚文思 刘香君 丁春慧 孙梦雪 秦园园 顾兵 燕宪亮 |
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作者单位: | 1. 徐州医学院,江苏 徐州,221004;2. 徐州医学院附属医院 检验科,江苏 徐州,221004;3. 徐州医学院附属医院 急救中心,江苏 徐州 221004; 徐州医学院附属医院 急诊医学实验室,江苏 徐州 221004 |
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摘 要: | 目的:研究苯妥英钠致骨质疏松作用及维生素D的预防作用。方法:将40只SD雄性大鼠随机分为4组(苯妥英钠模型组、D–半乳糖模型组、维生素D预防组、正常对照组),每组10只。其中,用苯妥英钠对苯妥英钠模型组及维生素D预防组大鼠连续灌胃60 d [54 mg/(kg?d)]。60 d后,对苯妥英钠模型组、D–半乳糖模型组、维生素D预防组和正常对照组的相关指标进行检测及比较组间差异。结果:苯妥英钠模型组骨钙[(592.87±120.89)μg]、磷[(173.88±15.98)μg]及血清钙[(2.65±0.09) mmol/L]、磷[(2.13±0.29) mmol/L]、超氧化物歧化酶[(104.40±3.58) NU/mgPro]含量均比正常对照组显著降低(P<0.01),血清碱性磷酸转移酶[(136.00±1.82) U/L]与正常对照组相比有显著升高(P<0.01),骨羟脯氨酸含量[(1128.974±460.77)μg],虽缺乏统计意义但相比于正常对照组仍有所降低,上述变化与D–半乳糖模型组的变化趋势相同,并比后者的变化更加显著。结论:长期大量摄入苯妥英钠可以导致大鼠发生骨质疏松的可能性增加,维生素D可以阻止或减缓这种变化趋势。
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关 键 词: | 苯妥英钠 骨质疏松症 维生素D D-半乳糖 |
Establishing osteoporosis model with phenytoin sodium in rats and investigating the preventive effects of vitamin D |
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Abstract: | Objective: To investigate the model of osteoporosis by phenytoin sodium and observe the preventive effects of vitamin D.Methods: A total of 40 SD male rats were randomly divided into phenytoin sodium model group, D–galactose model group, vitamin D group and normal control group (n=10 for each group). Phenytoin sodium model group and vitamin D group were given lavages of phenytoin sodium for 60 days [54 mg/(kg·d)]. Then routine osseous parameters were tested and compared among 4 groups.Results:Compared with normal control group, bone calcium [(592.87±120.89) μg], phosphorus [(173.88±15.98) μg] and serum calcium [(2.65±0.09) mmol/L], phosphorus [(2.13±0.29) mmol/L], SOD [(104.40±3.58) NU/mgPro] in the phenytoin sodium model group decreased signiifcantly (P<0.01). However, the serum ALP increased signiifcantly (P<0.01). At the same time, there was some decrease in the content of bone hydroxyproline. hTese changes above were the same as the D– galactose model group and were more outstanding.Conclusion:hTe high dose of phenytoin sodium intake can cause osteoporosis in rat, but vitamin D can prevent and inhibit osseous changes induced by phenytoin sodium. |
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Keywords: | phenytoin sodium osteoporosis vitamin D D-galactose |
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