苯妥英钠致大鼠骨质疏松模型的建立及维生素D预防作用的观察

目的：研究苯妥英钠致骨质疏松作用及维生素D的预防作用。方法：将40只SD雄性大鼠随机分为4组(苯妥英钠模型组、D–半乳糖模型组、维生素D预防组、正常对照组)，每组10只。其中，用苯妥英钠对苯妥英钠模型组及维生素D预防组大鼠连续灌胃60 d [54 mg/(kg?d)]。60 d后，对苯妥英钠模型组、D–半乳糖模型组、维生素D预防组和正常对照组的相关指标进行检测及比较组间差异。结果：苯妥英钠模型组骨钙[(592.87±120.89)μg]、磷[(173.88±15.98)μg]及血清钙[(2.65±0.09) mmol/L]、磷[(2.13±0.29) mmol/L]、超氧化物歧化酶[(104.40±3.58) NU/mgPro]含量均比正常对照组显著降低(P<0.01)，血清碱性磷酸转移酶[(136.00±1.82) U/L]与正常对照组相比有显著升高(P<0.01)，骨羟脯氨酸含量[(1128.974±460.77)μg]，虽缺乏统计意义但相比于正常对照组仍有所降低，上述变化与D–半乳糖模型组的变化趋势相同，并比后者的变化更加显著。结论：长期大量摄入苯妥英钠可以导致大鼠发生骨质疏松的可能性增加，维生素D可以阻止或减缓这种变化趋势。

Establishing osteoporosis model with phenytoin sodium in rats and investigating the preventive effects of vitamin D

Objective: To investigate the model of osteoporosis by phenytoin sodium and observe the preventive effects of vitamin D.Methods: A total of 40 SD male rats were randomly divided into phenytoin sodium model group, D–galactose model group, vitamin D group and normal control group (n=10 for each group). Phenytoin sodium model group and vitamin D group were given lavages of phenytoin sodium for 60 days [54 mg/(kg·d)]. Then routine osseous parameters were tested and compared among 4 groups.Results:Compared with normal control group, bone calcium [(592.87±120.89) μg], phosphorus [(173.88±15.98) μg] and serum calcium [(2.65±0.09) mmol/L], phosphorus [(2.13±0.29) mmol/L], SOD [(104.40±3.58) NU/mgPro] in the phenytoin sodium model group decreased signiifcantly (P<0.01). However, the serum ALP increased signiifcantly (P<0.01). At the same time, there was some decrease in the content of bone hydroxyproline. hTese changes above were the same as the D–
galactose model group and were more outstanding.Conclusion:hTe high dose of phenytoin sodium intake can cause osteoporosis in rat, but vitamin D can prevent and inhibit osseous changes induced by phenytoin sodium.