3D打印气管补片细胞相容性和生物力学性能研究

目的 研究3D打印气管补片的生物力学性能及其与骨髓间充质干细胞(BMSCs)的生物相容性.方法 从幼年新西兰大白兔胫骨平台抽取骨髓,通过全骨髓培养及贴壁纯化法获取BMSCs进行培养和传代.对3D打印气管补片进行生物力学测试;同时将其与BMSCs共培养后进行细胞形态观察,并用磺酰罗丹明B(SRB)比色法测定细胞增殖活性.结果 3D打印气管补片具有良好的生物力学性能;其与BMSCs共培养后细胞形态正常,贴壁生长良好,且SRB比色法显示细胞增殖良好.结论 3D打印气管补片具备良好的生物力学性能和生物相容性,是一种具有开发潜力的生物材料,可用于组织工程气管的体外构建.

Cellular biocompatibility and biomechanical properties of 3D printed tracheal graft

Objective To prepare 3D printed tracheal graft and investigate its cellular biocompatibility and biomechanical properties.Methods Bone marrow was isolated from tibial plateau of young New Zealand white rabbit,and bone mesenchymal stem cells (BMSCs) were obtained by whole bone marrow culture method and adherent purification method.Biomechanical test was performed for 3D printed trachea graft.After co-cultured of 3D printed trachea graft and BMSCs,cell morphology was observed and the proliferation index of the cells on 3D printed trachea graft was quantified using sulforhodamine B (SRB) assay.Results 3D printed trachea graft showed excellent biomechanical properties.Cell morphology was normal and cells grew well after co-culture with 3D printed trachea graft.The SRB assay indicated good proliferation of BMSCs on 3D printed trachea graft.Conclusions 3D printed trachea graft shows favorable cellular biocompatibility and biomechanical properties,and therefore can be used as a scaffold material for tissue-engineered trachea.