MMP16在胰腺癌组织中的表达及其临床意义

目的：检测胰腺癌组织中MMP16蛋白表达情况，探讨其在胰腺癌发生、发展中的作用、及其临床意义。方法：应用免疫印迹法检测8对胰腺癌/癌旁组织中MMP16蛋白的表达情况；应用免疫组化EnVision法检测49例胰腺癌组织中MMP16蛋白的表达情况，分析其与临床病理因素的关系。结果：免疫印迹法检测显示，胰腺癌组织MMP16蛋白的相对表达量均高于相应癌旁组织(8/8)；免疫组化检测显示，在胰腺癌组织中MMP16蛋白的表达率为85.7%(42/49)，而在癌旁正常组织中MMP16蛋白表达率为9.1%(3/33)；MMP16蛋白在癌组织中的表达率明显高于癌旁正常组织，差异有统计学意义(P<0.05)。MMP16表达与胰腺癌的组织分化程度、临床分期、淋巴结转移有关；该三因素的分组间均有统计学差异(P<0.05)。结论：MMP16在胰腺癌组织中呈高表达，因此可能在胰腺癌的发生、发展中起重要作用。

Expression and clinical significance of MMP16 in pancreatic carcinoma

Objective:To detect the expression of MMP16 and to investigate its roles in the formation, development and clinical signiifcance of human pancreatic carcinoma. Methods:Immunoblot was utilized to detect the expression of MMP16 in 8 paired pancreatic carcinoma and adjacent non-tumorous tissues;immunohistochemical staining was used to detect the expression of MMP16 in 49 pancreatic carcinoma tissues;the relationship between MMP16 and the clinical pathological factors was analyzed. Results:Immunoblot proved that the expression of MMP16 protein in pancreatic carcinoma was higher than that in matched adjacent non-tumorous tissues (8/8);immunohistochemical staining demonstrated that the expression rate of MMP16 in pancreatic carcinoma was 85.7%, significantly higher than that of normal group (9.1%), the difference was statistically significant (P<0.05). the positive expression of MMP16 was closely related to the degree of differentiation, clinical stages and lymph node metastasis There were significant differences among the groups (P<0.05). Conclusion: MMP16 is overexpressed in human pancreatic carcinoma, and may play an important role in the carcinogenesis and progress of pancreatic carcinoma.