基于脾胃—脑相关理论研究电针对溃疡性结肠炎大鼠大脑皮层代谢物的影响

目的依据＂脾胃—脑相关＂理论,研究足阳明经穴位电针疗法对溃疡性结肠炎（ulcerative colitis,UC）大鼠大脑皮层代谢物的影响,识别关键代谢物。方法采用葡聚糖硫酸钠（dextran sulfate sodium,DSS）法复制UC大鼠模型,并随机分为模型组、电针组,每组13只,另选取13只大鼠为空白对照组（空白组）。采用疏密波电针足三里、天枢和上巨虚穴5天后,所有动物处死取直结肠和回盲部行病理形态学观察;分离脑皮层,分别提取水溶性和脂溶性脑组织代谢物,运用1H-NMR技术进行代谢组学研究。结果电针能明显改善UC大鼠模型的一般状况,降低UC大鼠的疾病活动指数（DAI）值（P〈0.05）,减轻UC大鼠肠道黏膜炎性细胞浸润,稳定线粒体、内质网等细胞结构。代谢组学研究显示,UC大鼠模型脑皮质中谷氨酸、TC和VLDL含量明显降低,而丙氨酸、LDL明显升高;电针干预后乳酸、谷氨酸、TC和VLDL均升高,而丙氨酸和LDL含量降低,与空白组接近。结论采用足阳明经穴针刺对UC模型脑组织代谢物有一定影响,基于脾胃与脑相关理论针刺治疗UC有其特定的代谢组学物质基础和机制。

Effect of Electro-acupuncture on Metabolites in the Cerebral Cortex of Ulcerative Colitis Rats Based on PiNVei-Brain Related Theory

Objective To study the effect of electro-acupuncture （EA） at points along Foot Yangming Channel on metabolite of ulcerative colitis （UC） rats＇ cerebral cortex and to identify key metabolites by referring to Pi/Wei-brain related theory in Chinese medicine （CM）. Methods The UC rat model was set up by dextran sulfate sodium （DSS） method. Male SD rats were randomly divided into the model group and the EA group, 13 in each group. Another 13 rats were recruited as the blank control group. Rats in the blank control group and the model group received no EA. EA was performed at Zusanli （ST36）, Shangjuxu （ST37） ,and Tianshu （ST25） for 5 days by using disperse-dense wave. Then all rats were sacrificed. Their recto-colon and the ileocecal junction were pathomorphologically observed by light microscope and transmission electron microscope （TEM）. Cerebral cortexes were extracted. Water-soluble and lipid-soluble brain tissue metabolites were respectively extracted for metabolic research using 1H nuclear magnetic resonance （1H-NMR）. Results EA could obviously improve the general condition of UC model rats, decrease the value of DAI, reduce the infiltration of inflammatory cells in the intestinal tract, stabilize structures such as mitochondria, endoplasmic reticulum and so on （P 〈0.05）. 1HNMIR a-nalysis showed that in the model group, contents of glutamic acid, cholesterol, very low density lipoprotein （VLDL） in the pallium obviously decreased, while alanine and low density lipoprotein （LDL） significantly increased. After EA, levels of lactic acid, glutamJc acid, total cholesterol （TC）, and VLDL all increased, and levels of alanine and LDL decreased. All indices were approximate to those of the blank control group. Conclusion EA at Foot Yangming channel was found to have some effect on metabolites in the brain tissue of UC model rats, which had specific metabonomic material basis and mechanism based on the Pi/Wei-brain related theory.