Determination of Acrylamide Metabolite,Mercapturic Acld by High Performance Liquid Chromatography

A HPLC Assay was developed to identify and measure the metabolite of acrylamide,mercapturic acid,N-Acetyl-s-(propionamid)-cysteine(APC)in urine.O-phthalaldehyde(OPA)was utilized as a precolumn derivatizing agent in the assay.This derivative was isolatedwith a good selectivity by high performance liquid chromatography(HPLC)employingreversed phase ODS columns.The quantitation of the mercapturic acid derivative wasreproducible and with a detection limit of l pmol.The average coefficient of variation for theruns carried out on the same day was approximately 4.6％ at the range of 80-160μmol·L~(-1) ofAPC,and the mean analytical recovery from urine samples was 94％. The metabolite of urine of workers exposed to acrylamide was identified as N-acetyl-s-(propionamide)-cysteine by HPLC chromatography and fluorescence scan and HPLC-Massspectra.All results were identical with the authentic synthesized compound.