Ⅰ型与Ⅱ型子宫内膜癌细胞抗氧化能力的比较

 目的 比较Ⅰ型和Ⅱ型子宫内膜癌细胞对氧化应激的抵抗并探讨其机制。  方法  ① H₂O₂处理Ⅰ型和Ⅱ型子宫内膜癌细胞，噻唑蓝（MTT）法检测两型细胞的增殖水平；② 120μmol H₂O₂和800μmol H₂O₂分别处理Ⅰ型和Ⅱ型子宫内膜癌细胞，流式细胞仪检测细胞内活性氧（ROS）水平，酶标仪检测细胞内还原型谷胱甘肽（GSH）和氧化型谷胱甘肽（GSSG）水平，分光光度计检测细胞内超氧化物歧化酶（SOD）、过氧化氢酶（CAT）及谷胱甘肽过氧化物酶（GPx）活性 结果 在不进行处理的情况下，Ⅰ型子宫内膜癌细胞较Ⅱ型子宫内膜癌细胞ROS水平及CAT活性较低，GSH水平、GSH/GSSG比值及SOD和GPx活性较高（P＜0.01）。在氧化应激条件下，Ⅰ型子宫内膜癌细胞GSH水平及GSH/GSSG比值下降，SOD和CAT活性降低（P＜0.01）；Ⅱ型子宫内膜癌细胞GSH、GSSG水平明显升高，GSH/GSSG比值略有升高，SOD、CAT和GPx活性升高（P＜0.01）。  结论  Ⅱ型子宫内膜癌细胞与Ⅰ型子宫内膜癌细胞相比较，其本身ROS水平较高，细胞内抗氧化系统及抗氧化酶系统对氧化刺激可进行有效应答，细胞不易受到氧化损伤。

Antioxidant ability of type I and type II endometrial cancer cells

Objective To compare the ability of type Ⅰand type Ⅱ endometrium cancer cells for resisting oxidative stress and investigate its related mechanism Method  ①  TypeⅠand type Ⅱ endometrium cancer cells were challenged by H₂O₂, and cell proliferation was determined by the MTT assay. ② TypeⅠor type Ⅱ endometrium cancer cells were treated with 120μmol H₂O₂ or 800?μmol H₂O₂, and cellular ROS, GSH, and GSSG levels and SOD, CAT and GPx activities were determined.  Results Without H₂O₂, typeⅠ endometriumcancer cells, compared with the type Ⅱ endometrium cancer cells, had a lower ROS level and CAT activity and a higher GSH level, GSH/GSSG ratio, and SOD and GPx activities. Under oxidative stress, the ROS level increased in both typeⅠand type Ⅱ endometrium cancer cells. GSH level, GSH/GSSG ratio, and SOD and CAT activities were decreased in type Ⅰ endometrium cancer cells while GSH and GSSG levels, GSH/GSSG ratio, and SOD, CAT and GPx activities increased in type Ⅱ endometrium cancer cells.  Conclusion  Oxidative stress is higher in typeⅡendometrium cancer cells than in typeⅠendometrium cancer cells. The oxidation reductions ystem and anti- oxidase system in type Ⅱ endometrium cancer cells can effectively respond to oxidative stress, and cells can effectively resist oxidative stress.