胃电刺激诱导大鼠延髓孤束核和迷走神经运动背核c-fos蛋白表达

背景：胃电刺激（GES）可以调控胃慢波，但其作用机制尚不完全清楚。原癌基因蛋白c—fos的表达可作为神经元功能活动的标志物。目的：以c-fos蛋白的表达为观察指标，探讨GES调控胃肌电慢波的神经机制。方法：将雌性Wistar大鼠随机分为对照组和GES组。GES以控制胃慢波为准，持续1h。分别于刺激后0．5、1、2和5h处死大鼠，以免疫组化方法观察c—fos蛋白在延髓孤束核和迷走神经运动背核中的表达。结果：对照组延髓孤束核和迷走神经运动背核仅见微量c—fos蛋白表达，GES后0．5h其表达开始增强，1h时达高峰，以后逐渐减弱。结论：GES可以调控胃肌电慢波，GES后延髓孤束核和迷走神经运动背核神经元c-fos蛋白表达阳性提示迷走神经可能参与了该调控作用。

Expression of c-fos Protein in the Nucleus of Tractus Solitarius and Dorsal Motor Nucleus of Vagus after Gastric Electric Stimulation in Rats

Background: Gastric electric stimulation (GES) can entrain gastric slow wave, but its mechanism is not fully understood. The expression of proto-oncogene protein c-fos can be taken as a marker for activation of neurons. Aims: To invest-igate the neuromechanism of GES in modulating the gastric slow wave by observing the expression of c-fos protein. Methods: Female Wistar rats were randomly divided into control group and four GES groups. GES was performed to control gastric slow wave in GES groups, then the rats were sacrificed 0.5, 1, 2 and 5 hours after one hour-GES. The expression of c-fos protein in the nucleus of tractus solitarius (NTS) and dorsal motor nucleus of vagus (DMV) of the central bulb was observed by immunohistochemical method. Results: c-fos protein in NTS and DMV expressed weakly in the control group. Thirty minutes after GES, its expression increased progressively and reached the peak one hour after GES, but reduced gradually subsequently. Conclusions: GES can entrain gastric slow wave. The marked change of c-fos protein expression in neurons of NTS and DMV indicates that the vagus nerve may be involved in the modulatory effect.