Identification of polymorphisms in the constant region of IgG3: the missing mouse allotype |
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| Authors: | Sun, Lirong Luce, Milson J. Ren, Kangmei Ha, Hyunjung Burrows, Peter D. |
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| Affiliation: | Department of Microbiology, Division of Developmental and Clinical Immunology, University of Alabama at Birmingham Birmingham, AL 35294, USA |
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| Abstract: | We have identified DNA sequence polymorphisms in the C 3 genesof BALB/c and C57BL/6 mice. One of these results in a Ser Glyamino acid difference in CH1 at position 129 according to theWu and Kabat numbering system. There are three additional silentsubstitutions in the coding region and two polymorphic nucleotidesin the 3' untranslated region. According to standard nomenclaturein which alleles are numbered according to the order of theiridentification, these C3 alleles are designated lgh-8a and Igh-8brespectively. We also describe two polymerase chain reaction-basedassays that identify the allelic differences. |
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| Keywords: | polymerase chain reaction restriction site polymorphism |
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