酪氨酸激酶Syk在乳腺癌中的表达及其临床意义

目的 :探索脾酪氨酸激酶Syk基因表达与乳腺癌生成及转移的关系 ,以及与雌激素受体 (estrogenrecep tor,ER)、孕激素受体 (progestogenreceptor,PR)、p5 3、HER2 neu的关系。方法 :用半定量RT PCR检测 4 0例乳腺癌标本、正常乳腺组织及其 15例良性乳房纤维瘤组织中SykmRNA的表达 ,并用免疫组化方法检测 4 0例乳腺癌组织中ER、PR、p5 3、HER2 neu的表达的情况。结果 :所有正常乳腺组织都有Syk基因的表达 ,而 4 0例乳腺癌组织中只有 9例表达 ,正常乳腺与乳腺癌组织中Syk基因表达率有显著差异 (χ2 =4 7 4 ,P <0 0 5 ) ,且乳腺癌组织中SykmRNA含量比正常乳腺组织显著降低 (t=3 4 1,P <0 0 5 )。有淋巴结转移的 18例乳腺癌组织中 ,1例有Syk基因表达 ,有淋巴结转移的乳腺癌SykmRNA的表达率和表达水平显著降低 (χ2 =3 77,P <0 0 5 ,t=2 74 ,P <0 0 5 )。结论 :Syk基因的表达在抑制乳腺癌的生长及转移过程中可能起着重要的作用。

Expression of Tyrosine Kinase Syk in Breast Cancer and Their Clinical Significance

Objective: To evaluate the effects of the Syk mRNA expression in human breast cancer on tumor growth and metastasis,and the correlation of the Syk gene expression with ER,PR,P53,and HER2/neu. Methods:Using semi-RT-PCR,specimens from 40 breast cancer patients(tumor tissues,adjacent normal tissues),and 15 fibroadenoma were detected for the expression of the Syk gene and level of Syk mRNA.Meanwhile,ER,PR,P53,HER2/neu were detected in 40 tumor tissues from breast cancer with immunohistochemical staining. Results:Expression of the Syk gene was detected in all normal breast tissues.Unlike normal breast tissue,31 out of 40 breast cancer tissues did not show any detectable Syk mRNA expression,and there were significant differences in two groups(P<0.05).The level of Syk mRNA in the primary breast cancer tissues was significantly lower than that in the adjacent non-cancerous breast tissues and benign fibroadenoma breast tissues(P<0.05).Furthermore,only two breast cancer tissues in 18 patients with lymph node metastasis had the Syk mRNA expression.The Syk mRNA expression was negatively correlated to lymph node metastasis,HER2/neu protein expression(P<0.05). Conclusion: The expression of the Syk gene may play an important role in suppressing growth and metastasis of breast cancer.